Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243052
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampZoppi, Claudio Cesarpt_BR
dc.contributor.authorunicampLeite, Nayara de Carvalhopt_BR
dc.contributor.authorunicampBranco, Renato Chaves Soutopt_BR
dc.contributor.authorunicampPaula, Flávia Maria Moura dept_BR
dc.contributor.authorunicampBorck, Patricia Cristinept_BR
dc.contributor.authorunicampVettorazzi, Jean Franciescopt_BR
dc.contributor.authorunicampCarneiro, Everardo Magalhãespt_BR
dc.typeOutros documentospt_BR
dc.type.categoryResumopt_BR
dc.titleAltered mitochondrial metabolism is involved with increased amplifying pathways modulation of insulin secretion in protein malnourished obese micept_BR
dc.contributor.authorZoppi, C. C.pt_BR
dc.contributor.authorLeite, N. C.pt_BR
dc.contributor.authorBranco, R. C. S.pt_BR
dc.contributor.authorPaula, F. M. M.pt_BR
dc.contributor.authorBorck, P. C.pt_BR
dc.contributor.authorVettorazzi, J. F.pt_BR
dc.contributor.authorCarneiro, E. M.pt_BR
dc.subjectDieta com restrição de proteínaspt_BR
dc.subjectCamundongos obesospt_BR
dc.subjectInsulina - Secreçãopt_BR
dc.subjectCanais KATPpt_BR
dc.subjectDieta hiperlipídicapt_BR
dc.subject.otherlanguageKATP channelspt_BR
dc.subject.otherlanguageInsulin - Secretionpt_BR
dc.subject.otherlanguageDiet, Protein-restrictedpt_BR
dc.subject.otherlanguageDiet, High-fatpt_BR
dc.subject.otherlanguageMice, Obesept_BR
dc.description.abstractGlucose-induced insulin secretion (GIIS) stimulates dependent and independent mechanisms of KATP channels. Low protein and high-fat diets, during important stages of development favor obesity progress inducing several changes in pancreatic islets. The changes on the independent mechanisms of KATP channels, also called amplifying pathways, induced by the combination of these two treatments are unknown. Our aim was investigating the role of these pathways on GIIS modulation of malnourished obese mice. After weaning, 21 days old male C57BL-6 mice were randomly assigned into the control group (C) which received a normo-protein diet (14% protein) during 105 days; the control-high fat diet (HFD) (CH) received normo-protein diet for 45 days and after that was treated with a HFD (60% fat) for 60 days. The protein restricted R and RH groups were fed with a low protein diet (LPD) (6% protein), receiving the same HFD treatment. Insulin tolerance was assessed by euglycemic-hyperinsulinemic clamp. In order to investigate amplifying pathways control of insulin secretion, we incubated islets with glucose (G2.8; 11.1; 22.2 mM) in the presence of K+ (30 mM) and diazoxide (250 μm). Insulin content was measured by radioimmunoassay. Protein kinases A and C and mitochondrial metabolism were also assessed. HFD decreased glucose uptake (C=25.6±2.1/CH=15.9±2.36; R=50.0±5.1/RH=18.18±3.9 mg/kg/min). GIIS was higher in animals fed with HFD (G22.2 C=1.58±0.18/CH=3.0±0.3; R=1.08±0.12/RH= 1.8±0.3 ng/mL/hour/islet). The influence of amplifying pathways in insulin secretion was lower in R animals (G22.2 C=0.757±0.1; R=0.33± 0.09 ng/mL/hour/islet). There were no differences among C and CH in all glucose concentrations. Additionally, the role of amplifying pathways was higher in RH (G22.2 R=0.33±0.09; RH=1.67±0.22). Whereas PKA and PKC content were not altered, ATP production displayed a trend to be reduced in RH. Basal mitochondrial membrane potential and glutamate dehydrogenase (GDH) protein content were nearly 40 and 50% higher, respectively. Higher amplifying pathway modulation of insulin secretion of obese protein malnourished mice is associated with impaired mitochondrial function and GDH content. This could be one of the mechanisms reprogrammed by early protein malnutrition, since this effect does not appear in animals fed with a normal protein plus HFD.pt_BR
dc.relation.ispartofDiabetologia: clinical and experimental diabetes and metabolismpt_BR
dc.relation.ispartofabbreviationDiabetologiapt_BR
dc.publisher.cityNew York, NYpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherSpringerpt_BR
dc.date.issued2015pt_BR
dc.date.monthofcirculationSept.pt_BR
dc.identifier.citationAltered Mitochondrial Metabolism Is Involved With Increased Amplifying Pathways Modulation Of Insulin Secretion In Protein Malnourished Obese Mice. Springer, v. 58, p. S340-S340 SEP-2015.pt_BR
dc.language.isoengpt_BR
dc.description.volume58pt_BR
dc.description.issuenumber713pt_BR
dc.description.issuesupplement1pt_BR
dc.description.issuespecial1pt_BR
dc.description.firstpageS340pt_BR
dc.description.lastpageS340pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0012-186Xpt_BR
dc.identifier.eissn1432-0428pt_BR
dc.identifier.doi10.1007/s00125-015-3687-4pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007/s00125-015-3687-4pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2016-06-07T13:21:15Z-
dc.date.accessioned2016-06-07T13:21:15Z-
dc.description.provenanceMade available in DSpace on 2016-06-07T13:21:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243052-
dc.description.conferencenome51 Annual Meeting of the European Association for the Study of Diabetes (EASD)pt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000359820901402pt_BR
dc.creator.orcid0000-0002-2985-5235pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-6886-7693pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-3212-369Xpt_BR
dc.type.formEventopt_BR
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000359820901402.pdf148.8 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.