Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242890
Type: Artigo
Title: Purification, partial characterization and antimicrobial activity of lectin from Chenopodium quinoa seeds
Author: Pompeu, Dávia Guimarães
Mattioli, Marcelo Augusto
Ribeiro, Rosy Iara Maciel de Azambuja
Gonçalves, Daniel Bonoto
Magalhães, Juliana Teixeira de
Marangoni, Sérgio
Silva, José Antônio da
Granjeiro, Paulo Afonso
Abstract: A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.
A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool
Subject: Atividade antimicrobiana
Quinoa
Glicose
Plantas - Lectinas
Sementes
Country: Brasil
Editor: Sociedade Brasileira de Ciencia e Tecnologia de Alimentos
Citation: Purification, Partial Characterization And Antimicrobial Activity Of Lectin From Chenopodium Quinoa Seeds. Soc Brasileira Ciencia Tecnologia Alimentos, v. 35, p. 696-703 OCT-DEC-2015.
Rights: aberto
Identifier DOI: 10.1590/1678-457X.6823
Address: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400696
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos

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