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Type: Artigo de periódico
Title: Neutralization Of The Neuromuscular Inhibition Of Venom And Taipoxin From The Taipan (oxyuranus Scutellatus) By F(ab ') 2 And Whole Igg Antivenoms
Author: Herrera
Maria; de O Collaco
Rita de Cassia; Villalta
Mauren; Segura
Alvaro; Vargas
Mariangela; Wright
Christine E.; Paiva
Owen K.; Matainaho
Teatulohi; Jensen
Simon D.; Leon
Guillermo; Williams
David J.; Rodrigues-Simioni
Lea; Maria Gutierrez
Abstract: The neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action of taipoxin was also studied at 21 degrees C. The efficacy of the antivenoms was also assessed in an in vivo mouse model. Both antivenoms were effective in neutralizing the neuromuscular blocking activity in preincubation-type experiments. In experiments involving independent addition of venom and antivenoms, neutralization depended on the time interval between venom addition and antivenom application. When taipoxin was incubated for 5, 10 or 20 min at 21 degrees C, and antivenom added and temperature increased to 37 degrees C, neutralization was achieved only when the toxin was incubated for 5 or 10 min. The neutralization by the two antivenoms in an in vivo model showed that both whole IgG and F(ab')(2) antivenoms were effective in neutralizing lethality. Our findings highlight the very rapid action of taipan venom at the nerve terminal, and the poor capacity of antivenoms to revert neurotoxicity as the time interval between venom or taipoxin application and antivenom addition increased. Additionally the disparity between molecular masses of the active substances of the two antivenoms did not result in differences in neutralization. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Subject: Papua-new-guinea
Presynaptic Neurotoxin
Electrocardiographic Abnormalities
Phospholipases A(2)
Country: CLARE
Citation: Neutralization Of The Neuromuscular Inhibition Of Venom And Taipoxin From The Taipan (oxyuranus Scutellatus) By F(ab ') 2 And Whole Igg Antivenoms. Elsevier Ireland Ltd, v. 241, p. 175-183 Jan-2016.
Rights: embargo
Identifier DOI: 10.1016/j.toxlet.2015.11.020
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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