Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242711
Type: Artigo de periódico
Title: Sequential Optimization Approach For Prebiotic Galactooligosaccharides Synthesis By Pseudozyma Tsukubaensis And Pichia Kluyveri
Author: Cavalcante Fai
Ana Elizabeth; Resende Simiqueli
Ana Paula; Ghiselli
Gislaine; Pastore
Glaucia Maria
Abstract: Synthesis of the prebiotic galactooligosaccharides from lactose fermentation by Pseudozyma tsukubaensis and Pichia kluyveri was investigated. In this study, a sequential strategy of two experimental designs was used to optimize galactooligosaccharides production. First, Plackett-Burman screening design was applied to evaluate the effects of lactose, yeast extract, urea concentration, inoculum concentration, pH and agitation rate on the response of galactooligosaccharides yield synthesized by both yeasts. The preliminary Plackett-Burman design allowed the identification of the pH and urea concentration as statistically significant variables in the range studied. In order to find the optimal conditions for galactooligosaccharides synthesis, a central composite rotational design was employed. The independent variables were lactose, yeast extract and urea concentrations, and the response taken into account was galactooligosaccharides yield produced by P. tsukubaensis. The optimized condition for this prebiotic synthesis was: lactose, yeast extract and urea at concentrations of 26.0, 0.8 and 1.8 g/100 mL respectively. Under the optimized and validated conditions, the transgalactosylation activity of P. tsukubaensis resulted in GOS yield of 28.35 GOS (g)/100 g of lactose at 24 h, with a maximum GOS production of 73.71 g/L, and approximately 50 g/100 g of lactose hydrolysis. (C) 2015 Elsevier Ltd. All rights reserved.
Subject: Galacto-oligosaccharide Production
Beta-galactosidase
Enzymatic-synthesis
Lactose Hydrolysis
Lactobacillus
Singularis
Design
Cells
Milk
Country: AMSTERDAM
Editor: ELSEVIER SCIENCE BV
Citation: Sequential Optimization Approach For Prebiotic Galactooligosaccharides Synthesis By Pseudozyma Tsukubaensis And Pichia Kluyveri. Elsevier Science Bv, v. 63, p. 1214-1219 OCT-2015.
Rights: embargo
Identifier DOI: 10.1016/j.lwt.2015.04.064
Address: http://www.sciencedirect.com/science/article/pii/S0023643815003461
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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