Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Differentiation Of C57/bl6 Mice Bone Marrow Mononuclear Cells Into Early Endothelial Progenitors Cells In Different Culture Conditions
Author: Carneiro
Giane D.; Godoy
Juliana A. P.; Werneck
Claudio C.; Vicente
Cristina P.
Abstract: Endothelial progenitor cells (EPCs) can be isolated from bone marrow and characterized by the expression of cellular markers such as CD34, CD133, VEGFR2, CD31, and VE-Cadherin, by the uptake of acetylated low-density lipoprotein and by in vitro tube formation in tridimensional matrices. These cells are able to differentiate into mature endothelial cells and participate in the re-endothelization of damaged vessels. In this work, we tested different cultured media that can promote the proliferation and differentiation of mononuclear cells (MNCs) into early EPCs, with defined concentrations of growth factors and serum in order to establish a composition that may ensure us the reproducibility of our cultures. MNCs from mice bone marrow were cultivated using selective culture media containing DMEM or M199 supplemented with 10% FBS, VEGF, bFGF, and IGF, for 3, 7, and 14 days. Differentiation into early EPCs was analyzed using immunohistochemistry, FACS and western blotting and by functional parameters as uptake of ac-LDL, and formation of vessel-like structures. The cells cultivated with medium DMEM-M1 (DMEM plus VEGF, bFGF and IGF) expressed CD34, CD133, CD31, VEGFR2, and VE-Cadherin at all culture time-points with increased expression of these markers after 7 days. Only EPCs cultured for 30 days were able to form vessel-like structure. The uptake of ac-LDL was observed after 3, 7, 14, and 30 days, confirming the differentiation of mononuclear cells into early EPCs. DMEM-M1 was able to sustain MNCs proliferation and differentiation, increasing the expression of the characteristic EPC markers, allowing the expansion of early EPCs in culture in a similar way to that observed in commercial available media.
Subject: Umbilical-cord Blood
Par-1 Activation
Country: HOBOKEN
Citation: Differentiation Of C57/bl6 Mice Bone Marrow Mononuclear Cells Into Early Endothelial Progenitors Cells In Different Culture Conditions. Wiley-blackwell, v. 39, p. 1138-1150 OCT-2015.
Rights: fechado
Identifier DOI: 10.1002/cbin.10487
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
wos_000362790900007.pdf7.85 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.