Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242658
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampBenitez, Suzana Ulianpt_BR
dc.contributor.authorunicampCarneiro, Everardo Magalhãespt_BR
dc.contributor.authorunicampOliveira, Alexandre Leite Rodrigues dept_BR
dc.typeArtigopt_BR
dc.titleSynaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus modelpt_BR
dc.contributor.authorBenitez, Suzana Ulianpt_BR
dc.contributor.authorCarneiro, Everardo Magalhãespt_BR
dc.contributor.authorOliveira, Alexandre Leite Rodrigues dept_BR
dc.subjectMedula espinhalpt_BR
dc.subjectCélulas do corno anteriorpt_BR
dc.subjectTerminações pré-sinápticaspt_BR
dc.subjectCamundongos endogâmicos NODpt_BR
dc.subjectNeurônios motorespt_BR
dc.subjectDiabetes Mellituspt_BR
dc.subject.otherlanguageDiabetes Mellituspt_BR
dc.subject.otherlanguageMotor neuronspt_BR
dc.subject.otherlanguageMice, Inbred NODpt_BR
dc.subject.otherlanguageSpinal cordpt_BR
dc.subject.otherlanguagePresynaptic terminalspt_BR
dc.subject.otherlanguageAnterior horn cellspt_BR
dc.description.abstractHyperglycemia is the main cause of diabetic complications, contributing to a widespread degeneration of the nervous system. Nevertheless, the main focus has been the sensory neurons because of neuropathic pain, while the impairments associated with the spinal cord and motor deficits, mostly of those initiated at early stages of the disease, have been poorly investigated. In this way, the present study used the nonobese diabetic mouse model to evaluate the microenvironment around motoneurons at ventral horn of the spinal cord, following prolonged hyperglycemia. Adult female mice were divided into two groups: spontaneously diabetic (n=33) and nondiabetic (n=26). Mice were considered hyperglycemic when blood glucose surpassed 400mg/dL. Following 2weeks from that stage, part of the animals was euthanized and the lumbar intumescences were obtained and processed for immunohistochemistry and transmission electron microscopy. For immunohistochemistry, the antibodies used for integrated density of pixels quantification were anti-synaptophysin, anti-GFAP, and anti-Iba1. The functional analysis was monitored with the walking track test (CatWalk system) during 4weeks. The results revealed significant motor impairment in diabetic animals in comparison to the control group. Such loss of motor control correlated with a significant reduction in presynaptic terminals apposed to the motoneurons. Nevertheless, there were no significant changes in glial reaction in the spinal cord. Overall, the results herein revealed central nervous system changes at early stages of the disease that may in turn contribute to the motor deficit. Such changes open a new window of investigation in early stages of diabetes to better comprehend motor impairment as a long-term complication of the diseasept_BR
dc.relation.ispartofBrain and Behaviorpt_BR
dc.relation.ispartofabbreviationBrain behav.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherJohn Wiley & Sonspt_BR
dc.date.issued2015pt_BR
dc.date.monthofcirculationOct.pt_BR
dc.identifier.citationSynaptic Input Changes To Spinal Cord Motoneurons Correlate With Motor Control Impairments In A Type 1 Diabetes Mellitus Model. John Wiley & Sons Inc, v. 5, p. OCT-2015.pt_BR
dc.language.isoengpt_BR
dc.description.volume5pt_BR
dc.description.issuenumber10pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage9pt_BR
dc.rightsabertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn2162-3279pt_BR
dc.identifier.doi10.1002/brb3.372pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1002/brb3.372pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumberCNPq [300552/2013-9]pt
dc.description.sponsordocumentnumber2014/06892-3; 2015/15377-8pt_BR
dc.description.sponsordocumentnumber300552/2013-9; 300552/2013-0pt_BR
dc.date.available2016-06-07T13:19:19Z-
dc.date.accessioned2016-06-07T13:19:19Z-
dc.description.provenanceMade available in DSpace on 2016-06-07T13:19:19Z (GMT). No. of bitstreams: 1 wos_000363423500003.pdf: 2249966 bytes, checksum: 51487532ae7cd2363563de12c2433846 (MD5) Previous issue date: 2015 Bitstreams deleted on 2020-06-15T14:11:59Z: wos_000363423500003.pdf,en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242658-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordMotoneuronpt_BR
dc.subject.keywordNonobese diabetic mice modelpt_BR
dc.subject.keywordSynaptic terminalspt_BR
dc.subject.keywordVentral hornpt_BR
dc.identifier.source000363423500003-
dc.creator.orcid0000-0002-3782-5319pt_BR
dc.creator.orcid0000-0003-3212-369Xpt_BR
dc.creator.orcid0000-0003-4224-4575pt_BR
dc.type.formArtigo Originalpt_BR
dc.identifier.articleide00372pt_BR
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