Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242589
Type: Artigo de periódico
Title: α-amylase In Vaginal Fluid: Association With Conditions Favorable To Dominance Of lactobacillus
Author: Nasioudis
Dimitrios; Beghini
Joziani; Bongiovanni
Ann Marie; Giraldo
Paulo C.; Linhares
Iara M.; Witkin
Steven S.
Abstract: Vaginal glycogen is degraded by host -amylase and then converted to lactic acid by Lactobacilli. This maintains the vaginal pH at 4.5 and prevents growth of other bacteria. Therefore, host -amylase activity may promote dominance of Lactobacilli. We evaluated whether the -amylase level in vaginal fluid is altered in women with bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) and whether its concentration was associated with levels of lactic acid isomers and host mediators. Vaginal fluid was obtained from 43 women with BV, 50 women with VVC, and 62 women with no vulvovaginal disorders. Vaginal fluid concentrations of -amylase, secretory leukocyte protease inhibitor (SLPI), hyaluronan, hyaluronidase-1, -defensin, and elafin were measured by enzyme-linked immunosorbent assay (ELISA). Vaginal concentrations of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase (MMP) 8, and d- and l-lactic acid levels in these patients were previously reported. The median vaginal fluid -amylase level was 1.83 mU/mL in control women, 1.45 mU/mL in women with VVC, and 1.07 mU/mL in women with BV. Vaginal levels of -amylase were correlated with d-lactic acid (P = .003) but not with l-lactic acid (P > .05) and with SLPI (P < .001), hyaluronidase-1 (P < .001), NGAL (P = .001), and MMP-8 (P = .005). The exfoliation of glycogen-rich epithelial cells into the vaginal lumen by hyaluronidase-1 and MMP-8 may increase glycogen availability and promote -amylase activity. The subsequent enhanced availability of glycogen breakdown products would favor proliferation of Lactobacilli, the primary producers of d-lactic acid in the vagina. Concomitant production of NGAL and SLPI would retard growth of BV-related bacteria.
Subject: Leukocyte Protease Inhibitor
Bacterial Vaginosis
Genital-tract
Candida-albicans
Iron Requirement
Acquisition
Expression
Consistent
Microbiota
Women
Country: THOUSAND OAKS
Editor: SAGE PUBLICATIONS INC
Rights: embargo
Identifier DOI: 10.1177/1933719115581000
Address: http://rsx.sagepub.com/content/22/11/1393
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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