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Type: Artigo de periódico
Title: Microsatellite Markers Isolated From Cabomba Aquatica Sl (cabombaceae) From An Enriched Genomic Library
Author: Barbosa
Tiago D. M.; Trad
Rafaela J.; Bajay
Miklos M.; Amaral
Maria C. E.
Abstract: Premise of the study: Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Methods and Results: Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Virua population. Conclusions: The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.
Subject: Plant Sciences
Country: ST LOUIS
Citation: Microsatellite Markers Isolated From Cabomba Aquatica Sl (cabombaceae) From An Enriched Genomic Library. Botanical Soc Amer Inc, v. 3, p. NOV-2015.
Rights: aberto
Identifier DOI: 10.3732/apps.1500076
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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