Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242430
Type: Artigo de periódico
Title: Optimization Of Amine-rich Multilayer Thin Films For The Capture And Quantification Of Prostate-specific Antigen
Author: Polak
Roberta; Bradwell
Grinia M.; Gilbert
Jonathan B.; Danielsen
Scott; Beppu
Marisa M.; Cohen
Robert E.; Rubner
Michael F.
Abstract: It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL.
Subject: Polyelectrolyte Multilayers
Carbon Nanotubes
Drug-delivery
Poly(allylamine Hydrochloride)
Biomedical Applications
Silver Nanoparticle
Gold Nanoparticles
Cutoff Point
Cancer
Proteins
Country: WASHINGTON
Editor: AMER CHEMICAL SOC
Rights: fechado
Identifier DOI: 10.1021/acs.langmuir.5b00443
Address: http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.5b00443
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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