Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242419
Type: Artigo de periódico
Title: In Vitro And In Vivo Studies Of Novel Poly(d,l-lactic Acid), Superhydrophilic Carbon Nanotubes, And Nanohydroxyapatite Scaffolds For Bone Regeneration
Author: Siqueira
Idalia A. W. B.; Corat
Marcus Alexandre F.; Cavalcanti
Bruno das Neves; Ribeiro Neto
Wilson Alves; Martin
Airton Abrahao; Suman Bretas
Rosario Elida; Marciano
Fernanda Roberta; Lobo
Anderson Oliveira
Abstract: Poly(D,L-lactide acid, PDLLA) has been researched for scaffolds in bone regeneration. However, its hydrophobocity and smooth surface impedes its interaction with biological fluid and cell adhesion. To alter the surface characteristics, different surface Modification techniques have been developed to facilitate biological application. The present study compared two different routes to produce PDLLA/superhydrophilic vertically aligned carbon nanotubes:nanohydroxyapatite (PDLLA/VACNT-O:nHAp) scaffolds. For this, we used electrodeposition and immersion in simulated body fluid (SBF). Characterization by goniometry, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy confirmed the polymer modifications, the in vitro bioactivity, and biomineralization. Differential sunning calorimetry and thermal gravimetric analyses showed that the inclusion of VACNT-O:nHA probably acts as a nucleating agent increasing the crystallization rate in the neat PDLLA without structural alteration. Our results showed the formation of a dense nHAp layer on all scaffolds after 14 days of immersion in SBF solution; the most intense carbonated nHAp peaks observed in the PDLLA/VACNT-O:nHAp samples suggest higher calcium precipitation compared to the PDLLA control. Both cell viability and alkaline phosphatase assays showed favorable results, because no cytotoxic effects were present and all produced scaffolds were able to induce detectable mineralization. Bone defects were used to evaluate the bone regeneration; the confocal Raman and histological results confirmed high potential for bone applications: In vivo study showed that the PDLLA/VACNT-O:nHAp scaffolds mimicked the immature bone and induced bone remodeling: These findings indicate surface improvement and the applicability of this new nanobiomaterial for hone regenerative medicine.
Subject: Biodegradable Polymers
Surface Modification
Poly(l-lactic Acid)
Protein Adsorption
Lactide Composite
Cells
Hydroxyapatite
Growth
Tissue
Nanocomposites
Country: WASHINGTON
Editor: AMER CHEMICAL SOC
Rights: fechado
Identifier DOI: 10.1021/acsami.5b01066
Address: http://pubs.acs.org/doi/abs/10.1021/acsami.5b01066
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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