Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242347
Type: Artigo
Title: Vagotomy Ameliorates Islet Morphofunction And Body Metabolic Homeostasis In Msg-obese Rats
Author: Lubaczeuski, C.
Balbo, S. L.
Ribeiro, R. A.
Vettorazzi, J. F.
Santos-Silva, J. C.
Carneiro, E. M.
Bonfleur, M. L.
Abstract: The parasympathetic nervous system is important for beta-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic beta-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and beta-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and beta-cell area/pancreas section, respectively. Also, the beta-cell number per beta-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater beta-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats.
The parasympathetic nervous system is important for beta-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic beta-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and beta-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and beta-cell area/pancreas section, respectively. Also, the beta-cell number per beta-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater beta-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats
Subject: Homeostase
Obesidade
Células secretoras de insulina
Glicose
Sistema nervoso parassimpático
Vagotomia
Country: Brasil
Editor: Associação Brasileira de Divulgação Científica
Citation: Vagotomy Ameliorates Islet Morphofunction And Body Metabolic Homeostasis In Msg-obese Rats. Assoc Bras Divulg Cientifica, v. 48, p. 447-457 MAY-2015.
Rights: aberto
Identifier DOI: 10.1590/1414-431X20144340
Address: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500447
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos

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