Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242346
Type: Artigo
Title: Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets
Author: Lippo, Bruno Rodrigo da Silva
Batista, Thiago Martins
Rezende, Luiz Fernando de
Cappelli, Ana Paula
Camargo, Rafael Ludemann
Branco, Renato Chaves Souto
Sampaio, Helena Cristina Barbosa
Protzek, André Otávio Peres
Wanderley, Maria Inês
Arantes, Vanessa Cristina
Corat, Marcus Alexandre Finzi
Carneiro, Everardo Magalhães
Udrisar, Daniel Pedro
Wanderley, Almir Gonçalves
Ferreira, Fabiano
Abstract: Protein restriction in the early stages of life can result in several changes in pancreatic function. These alterations include documented reductions in insulin secretion and in cytoplasmic calcium concentration [Ca2+](i). However, the mechanisms underlying these changes have not been completely elucidated and may result, in part, from alterations in signaling pathways that potentiate insulin secretion in the presence of glucose. Our findings suggest that protein restriction disrupts the insulin secretory synergism between Cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and Ca2+ -dependent protein kinase C (PKC) in isolated islets. Western blot analysis demonstrated reduced levels of both phospho-cAMP response element-binding protein (phospho-CREB) at Ser-133 and substrates phosphorylated by PKCs (Phospho-(Ser) PKC substrate), suggesting that PICA and PKC activity was impaired in islets from rats fed a low-protein diet (LP). cAMP levels and global Ca2+ entry were also reduced in LP islets. In summary, our findings showed that protein restriction altered the crosstalk between PICA and PKC signaling pathways, resulting in the alteration of secretory synergism in isolated islets. (C) 2015 Elsevier Inc. All rights reserved.
Protein restriction in the early stages of life can result in several changes in pancreatic function. These alterations include documented reductions in insulin secretion and in cytoplasmic calcium concentration [Ca2+](i). However, the mechanisms underlying these changes have not been completely elucidated and may result, in part, from alterations in signaling pathways that potentiate insulin secretion in the presence of glucose. Our findings suggest that protein restriction disrupts the insulin secretory synergism between Cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and Ca2+ -dependent protein kinase C (PKC) in isolated islets. Western blot analysis demonstrated reduced levels of both phospho-cAMP response element-binding protein (phospho-CREB) at Ser-133 and substrates phosphorylated by PKCs (Phospho-(Ser) PKC substrate), suggesting that PICA and PKC activity was impaired in islets from rats fed a low-protein diet (LP). cAMP levels and global Ca2+ entry were also reduced in LP islets. In summary, our findings showed that protein restriction altered the crosstalk between PICA and PKC signaling pathways, resulting in the alteration of secretory synergism in isolated islets
Subject: Ilhotas pancreáticas
Insulina - Secreção
Dieta com restrição de proteínas
Diafonia
Proteína quinase A
Proteína quinase C
Country: Estados Unidos
Editor: Elsevier
Citation: Low-protein Diet Disrupts The Crosstalk Between The Pka And Pkc Signaling Pathways In Isolated Pancreatic Islets. Elsevier Science Inc, v. 26, p. 556-562 MAY-2015.
Rights: fechado
Identifier DOI: 10.1016/j.jnutbio.2014.12.010
Address: http://www.sciencedirect.com/science/article/pii/S0955286315000261
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos
CEMIB - Artigos e Outros Documentos

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