Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/24222
Type: Artigo de periódico
Title: Thiol-independent activity of a cholesterol-binding enterohemolysin produced by enteropathogenic Escherichia coli
Author: Figueirêdo, P.M.S.
Catani, C.F.
Yano, T.
Abstract: Enterohemolysin produced by Escherichia coli associated with infant diarrhea showed characteristics similar to those of thiol-activated hemolysins produced by Gram-positive bacteria, including inactivation by cholesterol, lytic activity towards eukaryotic cells and thermoinstability. However, enterohemolysin activity was not inactivated by oxidation or by SH group-blocking agents (1 mM HgCl2, 1 mM iodoacetic acid) and the hemolysin (100 µg/ml) was not lethal to mice, in contrast to the lethality of the thiol-activated hemolysin family to animals. Earlier reports showed that intravenous injection of partially purified streptolysin O preparations (0.2 µg) was rapidly lethal to mice. These results suggest that E. coli enterohemolysin is not a thiol-activated hemolysin, despite its ability to bind cholesterol, probably due to the absence of free thiol-group(s) that characterize the active form of the thiol-activated hemolysin molecule.
Subject: Escherichia coli
Enterohemolysin
Thiol-independent activity
Cholesterol binding
Editor: Associação Brasileira de Divulgação Científica
Rights: aberto
Identifier DOI: 10.1590/S0100-879X2003001100008
Address: http://dx.doi.org/10.1590/S0100-879X2003001100008
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100008
Date Issue: 1-Nov-2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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