Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242158
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMetabolic Screening For Pks And Nrps In Endophytic Actinobacteria From Citrus Reticulatapt_BR
dc.contributor.authorda Cruzpt_BR
dc.contributor.authorPedro L. R.; Giarolapt_BR
dc.contributor.authorLeila R.; Moraespt_BR
dc.contributor.authorSuellen da Silva; da Silvapt_BR
dc.contributor.authorDeborah Ellen S. G.; Marconpt_BR
dc.contributor.authorJoelma; Azevedopt_BR
dc.contributor.authorJoao L.; Araujopt_BR
dc.contributor.authorWelington L.; de Oliveirapt_BR
dc.contributor.authorLuciana G.pt_BR
unicamp.author.emailluciana@iqm.unicamp.brpt_BR
unicamp.authorda Silva, Deborah Ellen S. G.] Univ Estadual Campinas, Inst Quim, Dept Quim Organ, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.author[Giarola, Leila R.] Univ Estadual Campinas, Fac Farm, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.author.external[da Cruz, Pedro L. R.pt
unicamp.author.externalMoraes, Suellen da Silvapt
unicamp.author.external[Marcon, Joelmapt
unicamp.author.externalAzevedo, Joao L.pt
unicamp.author.externalde Oliveira, Luciana G.] Univ Sao Paulo, Escola Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP, Brazilpt
unicamp.author.external[Araujo, Welington L.] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, SP, Brazilpt
dc.subjectComplete Genome Sequencept_BR
dc.subjectAdenylation Domainspt_BR
dc.subjectPolyketide Synthasept_BR
dc.subjectBiosynthesispt_BR
dc.subjectActinomycetept_BR
dc.subjectSpecificitypt_BR
dc.subjectPathwayspt_BR
dc.subjectProductspt_BR
dc.subjectGeneticspt_BR
dc.subjectComplexpt_BR
dc.description.abstractPolyketides and non-ribosomal peptides are natural products widely found in bacteria, fungi and plants. The biological activities associated with these metabolites have attracted special attention in biopharmaceutical studies. Polyketide synthases act similarly to fatty acids synthetases and the whole multi-enzymatic set coordinating precursor and extending unit selection and reduction levels during chain growth. Acting in a similarly orchestrated model, non-ribosomal peptide synthetases biosynthesize NRPs. PKSs-I and NRPSs enzymatic modules and domains are collinearly organized with the parent gene sequence. This arrangement allows the use of degenerated PCR primers to amplify targeted regions in the genes corresponding to specific enzymatic domains such as ketosynthases and acyltransferases in PKSs and adenilation domains in NRPSs. Careful analysis of these short regions allows the classifying of a set of organisms according to their potential to biosynthesize PKs and NRPs. In this work, the biosynthetic potential of a set of 13 endophytic actinobacteria from Citrus reticulata for producing PKs and NRP metabolites was evaluated. The biosynthetic profile was compared to antimicrobial activity. Based on the inhibition promoted, 4 strains were considered for cluster analysis. A PKS/NRPS phylogeny was generated in order to classify some of the representative sequences throughout comparison with homologous genes. Using this approach, a molecular fingerprint was generated to help guide future studies on the most promising strains.en
dc.relation.ispartofQUIMICA NOVApt_BR
dc.publisher.countrySAO PAULOpt_BR
dc.publisherSOC BRASILEIRA QUIMICApt_BR
dc.date.issued2015-MARpt_BR
dc.identifier.citationMetabolic Screening For Pks And Nrps In Endophytic Actinobacteria From Citrus Reticulata. Soc Brasileira Quimica, v. 38, p. 333-341 MAR-2015.pt_BR
dc.language.isoptpt_BR
dc.description.volume38pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage333pt_BR
dc.description.lastpage341pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0100-4042pt_BR
dc.identifier.wosidWOS:000354781600008pt_BR
dc.identifier.doi10.5935/0100-4042.20150010pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300333&lng=en&nrm=iso&tlng=enpt_BR
dc.date.available2016-06-07T13:16:43Z-
dc.date.accessioned2016-06-07T13:16:43Z-
dc.description.provenanceMade available in DSpace on 2016-06-07T13:16:43Z (GMT). No. of bitstreams: 1 wos_000354781600008.pdf: 4794646 bytes, checksum: 1c2cecd84a63fbd557fb2bed6217d934 (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242158-
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