Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242137
Type: Artigo de periódico
Title: Molecular Detection Of The Blood Meal Source Of Sand Flies (diptera: Psychodidae) In A Transmission Area Of American Cutaneous Leishmaniasis, Parana State, Brazil
Author: Baum
Mauricio; de Castro
Edilene Alcantara; Pinto
Mara Cristina; Goulart
Thais Marchi; Baura
Walter; Klisiowicz
Debora do Rocio; Vieira da Costa-Ribeiro
Magda Clara
Abstract: The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the blood. (C) 2014 Elsevier B.V. All rights reserved.
Subject: Nyssomyia-intermedia
Feeding Preference
Cytochrome-b
Minas-gerais
Endemic Area
Identification
Phlebotominae
Reservoirs
Pcr
Emphasis
Country: AMSTERDAM
Editor: ELSEVIER SCIENCE BV
Rights: embargo
Identifier DOI: 10.1016/j.actatropica.2014.11.006
Address: http://www.sciencedirect.com/science/article/pii/S0001706X14003593
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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