Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242084
Type: Artigo
Title: Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats
Author: Salvatierra, Cristiana S. B.
Reis, Sílvia R. L.
Pessoa, Ana F. M.
Souza, Letícia M. I. de
Stoppiglia, Luiz F.
Veloso, Roberto V.
Reis, Marise A. B.
Carneiro, Everardo M.
Boschero, Antonio C.
Colodel, Edson M.
Arantes, Vanessa C.
Latorraca, Márcia Q.
Abstract: The phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways mediate beta cell growth, proliferation, survival and death. We investigated whether protein restriction during pregnancy alters islet morphometry or the expression and phosphorylation of several proteins involved in the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways. As controls, adult pregnant and non-pregnant rats were fed a normal-protein diet (17%). Pregnant and non-pregnant rats in the experimental groups were fed a low-protein diet (6%) for 15 days. Low protein diet during pregnancy increased serum prolactin level, reduced serum corticosterone concentration and the expression of both protein kinase B/AKT1 (AKT1) and p70 ribosomal protein S6 kinase (p70(S6K)), as well as the islets area, but did not alter the insulin content of pancreatic islets. Pregnancy increased the expression of the Src homology/collagen (SHC) protein and the extracellular signal-regulated kinases 1/2 (ERK1/2) independent of diet. ERK1/2 phosphorylation (pERK1/2) was similar in islets from pregnant and non-pregnant rats fed a low-protein diet, and was higher in islets from pregnant rats than in islets from non-pregnant rats fed a normal-protein diet. Thus, a short-term, low-protein diet during pregnancy was sufficient to reduce the levels of proteins in the phosphatidylinositol 3-kinase pathway and affect islet morphometry.
The phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways mediate beta cell growth, proliferation, survival and death. We investigated whether protein restriction during pregnancy alters islet morphometry or the expression and phosphorylation of several proteins involved in the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways. As controls, adult pregnant and non-pregnant rats were fed a normal-protein diet (17%). Pregnant and non-pregnant rats in the experimental groups were fed a low-protein diet (6%) for 15 days. Low protein diet during pregnancy increased serum prolactin level, reduced serum corticosterone concentration and the expression of both protein kinase B/AKT1 (AKT1) and p70 ribosomal protein S6 kinase (p70(S6K)), as well as the islets area, but did not alter the insulin content of pancreatic islets. Pregnancy increased the expression of the Src homology/collagen (SHC) protein and the extracellular signal-regulated kinases 1/2 (ERK1/2) independent of diet. ERK1/2 phosphorylation (pERK1/2) was similar in islets from pregnant and non-pregnant rats fed a low-protein diet, and was higher in islets from pregnant rats than in islets from non-pregnant rats fed a normal-protein diet. Thus, a short-term, low-protein diet during pregnancy was sufficient to reduce the levels of proteins in the phosphatidylinositol 3-kinase pathway and affect islet morphometry
metadata.dc.description.abstractalternative: As vias da fosfatidilinositol-3-cinase e da proteína cinase ativada por mitógeno medeiam crescimento, proliferação, sobrevivência e morte das células β. Investigamos se a restrição proteica durante a prenhez altera a morfometria da ilhota ou a expressão e a fosforilação de diversas proteínas envolvidas nas vias da fosfatidilinositol-3-cinase e da proteína cinase ativada por mitógeno. Como controles, ratas adultas não prenhes e prenhes foram alimentadas com uma dieta norpoproteica (17%). As ratas não prenhes e prenhes dos grupos experimentais foram alimentadas com dieta hipoproteica (6%) por 15 dias. A dieta hipoproteica durante a prenhez aumentou as concentrações séricas de prolactina, reduziu as concentrações séricas de corticosterona e a expressão da proteína cinase B/ AKT1 (AKT1) e da proteína p70 ribossomal S6 cinase (p70S6K), bem como a área das ilhotas, mas não alterou o conteúdo de insulina nas ilhotas pancreáticas. A prenhez aumentou a expressão da proteína homóloga ao colágeno com domínio SH2 (SHC) e a proteína cinase regulada por sinal extracelular 1/2 (ERK1/2), independente da dieta consumida. A fosforilação da ERK1/2 (pERK1/2) foi similar em ilhotas de ratas prenhes e não prenhes alimentadas com dieta hipoproteica, e foi maior em ilhotas de ratas prenhes do que não prenhes alimentadas com dieta normoproteica. Portanto, um curto período de dieta hipoproteica durante a prenhez foi suficiente para reduzir o conteúdo de proteínas da via da fosfatidilinositol-3-cinase e afetar a morfometria da ilhota
Subject: Sistema de sinalização das MAP quinases
Ilhotas pancreáticas
Fosfatidilinositol 3-quinase
Prenhez
Dieta com restrição de proteínas
Rato
Country: Brasil
Editor: Academia Brasileira de Ciências
Citation: Short-term Low-protein Diet During Pregnancy Alters Islet Area And Protein Content Of Phosphatidylinositol 3-kinase Pathway In Rats. Acad Brasileira De Ciencias, v. 87, p. 1007-1018 JUN-2015.
Rights: aberto
Identifier DOI: 10.1590/0001-3765201520140251
Address: http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0001-37652015000201007
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos

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