Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.typeArtigo de periódicopt_BR
dc.titleGenetic Diversity Of The Mycobacterium Tuberculosis Beijing Family In Brazil And Mozambique And Relation With Infectivity And Induction Of Necrosis In Thp-1 Cellspt_BR
dc.contributor.authorLia Lima; Goncalves Vasconcellospt_BR
dc.contributor.authorSidra Ezidrio; Gomespt_BR
dc.contributor.authorHarrison Magdinier; Eliaspt_BR
dc.contributor.authorAtina Ribeiro; Rochapt_BR
dc.contributor.authorAdalgiza da Silva; Ribeiropt_BR
dc.contributor.authorSimone C. M.; Panuntopt_BR
dc.contributor.authorAlessandra Costa; Ferrazolipt_BR
dc.contributor.authorLucilaine; da Silva Tellespt_BR
dc.contributor.authorMaria Alice; Emanuel Ivens dept_BR
dc.contributor.authorAraujo Marelo; Kritskipt_BR
dc.contributor.authorAfranio Lineu; Mokrousovpt_BR
dc.contributor.authorIgor; Manichevapt_BR
dc.contributor.authorOlga A.; Lasunskaiapt_BR
dc.contributor.authorElena; Suffyspt_BR
dc.contributor.authorPhilip Noelpt_BR[Panunto, Alessandra Costa] Univ Estadual Campinas, Lab Bacterial Pathogenesis & Mol Biol, Sao Paulo, Brazilpt_BR[Gomes, Lia Limapt Vasconcellos, Sidra Ezidriopt, Harrison Magdinierpt, Atina Ribeiropt, Adalgiza da Silvapt Ivens de, Araujo Marelopt, Philip Noel] Inst Oswaldo Cruz, Lab Mol Biol Appl Mycobacteria, BR-20001 Rio De Janeiro, Brazilpt[Ribeiro, Simone C., Elena] Univ Estadual Norte Fluminense, Lab Biol Recognit, Rio De Janeiro, Brazilpt[Ferrazoli, Lucilainept Silva Telles, Maria Alice] Adolfo Lutz Inst, Sao Paulo, Brazilpt[Kritski, Afranio Lineu] Univ Fed Rio de Janeiro, Univ Hosp Clementino Fraga Filho, Lab Micobacteriol Mol, Ctr Pesquisas Doencas Infecciosas & Parasitarias, Rio de Janeiro, Brazilpt[Mokrousov, Igor] St Petersburg Pasteur Inst, Mol Microbiol Lab, St Petersburg, Russiapt[Manicheva, Olga A.] Res Inst Phthisiopulmonol, Microbiol Lab, St Petersburg, Russiapt
dc.description.abstractIntroduction: The success of the Mycobacterium tuberculosis Beijing (MtbB) lineage in different geographical regions has been attributed to high transmission, increased virulence, drug resistance and rapid adaptation to the host. In some countries of secondary MtbB dispersion like South Africa and Peru, rising prevalence of the Beijing strains is registered. However, in neighboring countries to affected regions such as Mozambique and Brazil, respectively, the prevalence of these strains is still low and this could be due to biological particularities of the circulating MtbB strains and/or differentiated host susceptibility. Objective: To characterize genetically and phenotypically MtbB strains isolated in Brazil (n = 8) and Mozambique (n = 17). Methods: This is a descriptive study of genotypes of the MtbB isolates, determined by spoligotyping, MIRU-VNTR typing, analysis of the IS6110 copy number in the NTF region and screening for mutations in mutT2, mutT4, rpoB, katG and pks 15/1 genes. Virulence-associated properties of the studied isolates were verified in the in vitro model of infection of human THP-1 cells. Results: The genotypes defined by the 24VNTRs were distinct for all isolates included in this study and presented an HGDI of 0.997. The VNTR patterns with seven copies of MIRU26 and seven copies of QUB26, representative for the previously described MtbB genotype B0, dominant in Russia, were detected in 38.5% of the studied isolates. In addition, all isolates presented RD105 deletion and a 7 bp insertion in pks15/1 gene. Almost all tested strains belonged to the RD181 sublineage, with the exception of two strains from Mozambique of RD150 sublineage. Combined analysis of the NTF region integrity and mutations in mutT genes showed that 62.5% and 47% of isolates obtained in Brazil and Mozambique, respectively, were of the ancestral genotype. The virulence index of the ancient isolates, evaluated in the THP-1 cells, was significantly lower than that of the modern genotype group. Conclusions: These data demonstrate genotype particularities of the Beijing strains isolated in Brazil and Mozambique, two countries of low prevalence of the MtbB lineage in local Mtb populations. In contrast to the neighboring countries with high prevalence of the MtbB strains of modern sublineage, significant proportions of the isolates obtained in Brazil and Mozambique were presented by the strains of the ancient sublineage. Our data suggest that lower virulence of the ancient strains, compared with the modern strains, could be involved in the slow spread of the MtbB strains in some regions. (C) 2015 Elsevier Ltd. All rights reserved.en
dc.identifier.citationGenetic Diversity Of The Mycobacterium Tuberculosis Beijing Family In Brazil And Mozambique And Relation With Infectivity And Induction Of Necrosis In Thp-1 Cells. Churchill Livingstone, v. 95, p. S190-S196 JUN-2015.pt_BR
dc.description.sponsorshipRussian Science Foundation [14-14-00292]pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.provenanceMade available in DSpace on 2016-06-07T13:16:13Z (GMT). No. of bitstreams: 1 wos_000357931300032.pdf: 365586 bytes, checksum: 04800f8c1f1ef7b141b58161326a50e1 (MD5) Previous issue date: 2015en
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
wos_000357931300032.pdf357.02 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.