Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242031
Type: Artigo de periódico
Title: Genetic Diversity Of The Mycobacterium Tuberculosis Beijing Family In Brazil And Mozambique And Relation With Infectivity And Induction Of Necrosis In Thp-1 Cells
Author: Gomes
Lia Lima; Goncalves Vasconcellos
Sidra Ezidrio; Gomes
Harrison Magdinier; Elias
Atina Ribeiro; Rocha
Adalgiza da Silva; Ribeiro
Simone C. M.; Panunto
Alessandra Costa; Ferrazoli
Lucilaine; da Silva Telles
Maria Alice; Emanuel Ivens de
Araujo Marelo; Kritski
Afranio Lineu; Mokrousov
Igor; Manicheva
Olga A.; Lasunskaia
Elena; Suffys
Philip Noel
Abstract: Introduction: The success of the Mycobacterium tuberculosis Beijing (MtbB) lineage in different geographical regions has been attributed to high transmission, increased virulence, drug resistance and rapid adaptation to the host. In some countries of secondary MtbB dispersion like South Africa and Peru, rising prevalence of the Beijing strains is registered. However, in neighboring countries to affected regions such as Mozambique and Brazil, respectively, the prevalence of these strains is still low and this could be due to biological particularities of the circulating MtbB strains and/or differentiated host susceptibility. Objective: To characterize genetically and phenotypically MtbB strains isolated in Brazil (n = 8) and Mozambique (n = 17). Methods: This is a descriptive study of genotypes of the MtbB isolates, determined by spoligotyping, MIRU-VNTR typing, analysis of the IS6110 copy number in the NTF region and screening for mutations in mutT2, mutT4, rpoB, katG and pks 15/1 genes. Virulence-associated properties of the studied isolates were verified in the in vitro model of infection of human THP-1 cells. Results: The genotypes defined by the 24VNTRs were distinct for all isolates included in this study and presented an HGDI of 0.997. The VNTR patterns with seven copies of MIRU26 and seven copies of QUB26, representative for the previously described MtbB genotype B0, dominant in Russia, were detected in 38.5% of the studied isolates. In addition, all isolates presented RD105 deletion and a 7 bp insertion in pks15/1 gene. Almost all tested strains belonged to the RD181 sublineage, with the exception of two strains from Mozambique of RD150 sublineage. Combined analysis of the NTF region integrity and mutations in mutT genes showed that 62.5% and 47% of isolates obtained in Brazil and Mozambique, respectively, were of the ancestral genotype. The virulence index of the ancient isolates, evaluated in the THP-1 cells, was significantly lower than that of the modern genotype group. Conclusions: These data demonstrate genotype particularities of the Beijing strains isolated in Brazil and Mozambique, two countries of low prevalence of the MtbB lineage in local Mtb populations. In contrast to the neighboring countries with high prevalence of the MtbB strains of modern sublineage, significant proportions of the isolates obtained in Brazil and Mozambique were presented by the strains of the ancient sublineage. Our data suggest that lower virulence of the ancient strains, compared with the modern strains, could be involved in the slow spread of the MtbB strains in some regions. (C) 2015 Elsevier Ltd. All rights reserved.
Subject: East-asia
Strains
Genotype
Population
Mutations
Differentiation
Sublineage
Thailand
Ancient
Systems
Country: EDINBURGH
Editor: CHURCHILL LIVINGSTONE
Citation: Genetic Diversity Of The Mycobacterium Tuberculosis Beijing Family In Brazil And Mozambique And Relation With Infectivity And Induction Of Necrosis In Thp-1 Cells. Churchill Livingstone, v. 95, p. S190-S196 JUN-2015.
Rights: embargo
Identifier DOI: 10.1016/j.tube.2015.02.025
Address: http://www.sciencedirect.com/science/article/pii/S1472979215000268
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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