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Type: Artigo de periódico
Title: Single Cell Oil Producing Yeasts Lipomyces Starkeyi And Rhodosporidium Toruloides: Selection Of Extraction Strategies And Biodiesel Property Prediction
Author: Bonturi
Nemailla; Matsakas
Leonidas; Nilsson
Robert; Christakopoulos
Paul; Miranda
Everson Alves; Berglund
Kris Arvid; Rova
Abstract: Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.
Subject: Microbial Lipid Production
Oleaginous Yeast
Rapid Method
Country: BASEL
Editor: MDPI AG
Rights: aberto
Identifier DOI: 10.3390/en8065040
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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