Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/24188
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampPereira, Luis Antonio Violin Diaspt_BR
dc.typeArtigopt_BR
dc.titleEthidium bromide-induced demyelination of the sciatic nerve of adult Wistar ratspt_BR
dc.contributor.authorRiet-Correa, G.pt_BR
dc.contributor.authorFernandes, C.G.pt_BR
dc.contributor.authorPereira, L.A.V.pt_BR
dc.contributor.authorGraça, D.L.pt_BR
dc.subjectDesmielinizaçãopt_BR
dc.subjectNervo isquiáticopt_BR
dc.subjectEtídiopt_BR
dc.subject.otherlanguageDemyelinationpt_BR
dc.subject.otherlanguageSciatic nervept_BR
dc.subject.otherlanguageEthidiumpt_BR
dc.description.abstractPeripheral nerve ultrastructure was assessed after single or multiple local injections of the intercalating dye ethidium bromide. Thirty-four adult Wistar rats of both sexes were divided into five groups and maintained in a controlled environment with rat chow and water ad libitum throughout the experiment. The experimental animals were injected with 1 µl of 0.1% ethidium bromide in 0.9% saline into the central third of the left sciatic nerve 1 (group 1), 2 (group 2), 4 (group 3), 6 (group 4) or 8 (group 5) times. In groups 2 to 5 the injections were made at 28-day intervals. Control animals received the same amount of 0.9% saline. The animals were killed at different times after injection: group 1 at 7 days (2 rats) and 15 days (2 rats); for groups 2, 3, 4 and 5, all rats were killed 10 days after the last injection and the lesions were investigated by light and transmission electron microscopy. In the acute lesions, intoxicated Schwann cells showed a vacuolated cytoplasm and separation of the sheaths from the axon. Myelin sheaths underwent progressive vesiculation and subsequent segmental demyelination. Myelin debris were withdrawn by macrophages and remyelination by Schwann cells was prominent. With the increase in the number of injections collagen fibers also increased in number and progressively enveloped smaller numbers of remyelinated axons composing new fascicles. Wallerian degeneration of fibers apparently not affected by ethidium bromide was more intense in the nerves from groups 4 and 5. The peripheral nerve repairs itself after demyelinating challenges with a profusion of collagen fibers and new fasciculations. This experimental model is valid to mimic recurrent demyelinating neuropathies.en
dc.description.abstractPeripheral nerve ultrastructure was assessed after single or multiple local injections of the intercalating dye ethidium bromide. Thirty-four adult Wistar rats of both sexes were divided into five groups and maintained in a controlled environment with rat chow and water ad libitum throughout the experiment. The experimental animals were injected with 1 µl of 0.1% ethidium bromide in 0.9% saline into the central third of the left sciatic nerve 1 (group 1), 2 (group 2), 4 (group 3), 6 (group 4) or 8 (group 5) times. In groups 2 to 5 the injections were made at 28-day intervals. Control animals received the same amount of 0.9% saline. The animals were killed at different times after injection: group 1 at 7 days (2 rats) and 15 days (2 rats)pt
dc.description.abstractfor groups 2, 3, 4 and 5, all rats were killed 10 days after the last injection and the lesions were investigated by light and transmission electron microscopy. In the acute lesions, intoxicated Schwann cells showed a vacuolated cytoplasm and separation of the sheaths from the axon. Myelin sheaths underwent progressive vesiculation and subsequent segmental demyelination. Myelin debris were withdrawn by macrophages and remyelination by Schwann cells was prominent. With the increase in the number of injections collagen fibers also increased in number and progressively enveloped smaller numbers of remyelinated axons composing new fascicles. Wallerian degeneration of fibers apparently not affected by ethidium bromide was more intense in the nerves from groups 4 and 5. The peripheral nerve repairs itself after demyelinating challenges with a profusion of collagen fibers and new fasciculations. This experimental model is valid to mimic recurrent demyelinating neuropathies.pt
dc.description.abstractPeripheral nerve ultrastructure was assessed after single or multiple local injections of the intercalating dye ethidium bromide. Thirty-four adult Wistar rats of both sexes were divided into five groups and maintained in a controlled environment with rat chow and water ad libitum throughout the experiment. The experimental animals were injected with 1 µl of 0.1% ethidium bromide in 0.9% saline into the central third of the left sciatic nerve 1 (group 1), 2 (group 2), 4 (group 3), 6 (group 4) or 8 (group 5) times. In groups 2 to 5 the injections were made at 28-day intervals. Control animals received the same amount of 0.9% saline. The animals were killed at different times after injection: group 1 at 7 days (2 rats) and 15 days (2 rats); for groups 2, 3, 4 and 5, all rats were killed 10 days after the last injection and the lesions were investigated by light and transmission electron microscopy. In the acute lesions, intoxicated Schwann cells showed a vacuolated cytoplasm and separation of the sheaths from the axon. Myelin sheaths underwent progressive vesiculation and subsequent segmental demyelination. Myelin debris were withdrawn by macrophages and remyelination by Schwann cells was prominent. With the increase in the number of injections collagen fibers also increased in number and progressively enveloped smaller numbers of remyelinated axons composing new fascicles. Wallerian degeneration of fibers apparently not affected by ethidium bromide was more intense in the nerves from groups 4 and 5. The peripheral nerve repairs itself after demyelinating challenges with a profusion of collagen fibers and new fasciculations. This experimental model is valid to mimic recurrent demyelinating neuropathies.pt_BR
dc.relation.ispartofBrazilian journal of medical and biological researchpt_BR
dc.relation.ispartofabbreviationBraz. j. med. biol. res.pt_BR
dc.publisher.cityRibeirão Preto, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherAssociação Brasileira de Divulgação Científicapt_BR
dc.date.issued2002pt_BR
dc.date.monthofcirculationJan.pt_BR
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 99-104, 2002.pt_BR
dc.language.isoengpt_BR
dc.description.volume35pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage99pt_BR
dc.description.lastpage104pt_BR
dc.rightsAbertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.eissn1414-431Xpt_BR
dc.identifier.doi10.1590/S0100-879X2002000100014 pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100014pt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTIFÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipFAPERGS - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO RIO GRANDE DO SULpt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTIFÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1FAPERGS - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO RIO GRANDE DO SULpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2014-07-17T15:05:01Z
dc.date.available2015-11-26T11:25:25Z-
dc.date.accessioned2014-07-17T15:05:01Z
dc.date.accessioned2015-11-26T11:25:25Z-
dc.description.provenanceMade available in DSpace on 2014-07-17T15:05:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-01-01 Bitstreams deleted on 2020-06-15T14:07:46Z: S0100-879X2002000100014.pdf,en
dc.description.provenanceMade available in DSpace on 2015-11-26T11:25:25Z (GMT). No. of bitstreams: 2 S0100-879X2002000100014.pdf: 163345 bytes, checksum: 16f028326bf920d9efa6c273652e7946 (MD5) S0100-879X2002000100014.pdf.txt: 18935 bytes, checksum: 90b6fd1ea1457fe50de1ece1a647b342 (MD5) Previous issue date: 2002-01-01en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/24188
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/24188-
dc.contributor.departmentDepartamento de Histologia e Embriologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordRemyelinationpt_BR
dc.subject.keywordEthidium bromidept_BR
dc.identifier.sourceS0100-879X2002000100014-
dc.creator.orcid0000-0002-9332-7285pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.type.formArtigo de pesquisapt_BR
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