Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/241843
Type: Artigo de periódico
Title: Biomarker Evaluation In Fish After Prolonged Exposure To Nano-tio2: Influence Of Illumination Conditions And Crystal Phase
Author: Clemente
Z.; Castro
V. L.; Feitosa
L. O.; Lima
R.; Jonsson
C. M.; Maia
A. H. N.; Fraceto
L. F.
Abstract: In this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm(2)/h). The following oxidative stress biomarkers were monitored: concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Other biomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothionein levels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols.
Subject: Titanium-dioxide Nanoparticles
Trout Oncorhynchus-mykiss
Zebrafish Danio-rerio
Oxidative Dna-damage
Tio2 Nanoparticles
Rainbow-trout
In-vitro
Aquatic Organisms
Daphnia-magna
Stress
Country: VALENCIA
Editor: AMER SCIENTIFIC PUBLISHERS
Citation: Biomarker Evaluation In Fish After Prolonged Exposure To Nano-tio2: Influence Of Illumination Conditions And Crystal Phase. Amer Scientific Publishers, v. 15, p. 5424-5433 JUL-2015.
Rights: fechado
Identifier DOI: 10.1166/jnn.2015.10021
Address: http://www.ncbi.nlm.nih.gov/pubmed/26373153
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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