Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/241835
Type: Artigo de periódico
Title: Improvement On Sugar Cane Bagasse Hydrolysis Using Enzymatic Mixture Designed Cocktail
Author: Bussamra
Bianca Consorti; Freitas
Sindelia; da Costa
Aline Carvalho
Abstract: The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and beta-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% +/- 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification. (C) 2015 Elsevier Ltd. All rights reserved.
Subject: Alpha-l-arabinofuranosidase
Trichoderma-reesei
Cellulase
Biomass
Proteins
Country: OXFORD
Editor: ELSEVIER SCI LTD
Rights: embargo
Identifier DOI: 10.1016/j.biortech.2015.03.117
Address: http://www.sciencedirect.com/science/article/pii/S0960852415004496
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
wos_000353193200024.pdf805.95 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.