Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/241713
Type: Artigo de periódico
Title: Electrochemical Detection Of Dengue Virus Ns1 Protein With A Poly(allylamine)/carbon Nanotube Layered Immunoelectrode
Author: Silva
Mizia M. S.; Dias
Ana C. M. S.; Silva
Barbara V. M.; Gomes-Filho
Sergio L. R.; Kubota
Lauro T.; Goulart
Marilia O. F.; Dutra
Rosa F.
Abstract: BACKGROUNDA sensitive nanostructured immunoelectrode based on poly(allylamine) (PAH) sandwich is developed for non-structural 1 (NS1) of dengue virus. NS1 is a secretory protein abundant in the acute phase of disease associated to hemorrhagic fever. Anti-NS1 antibodies are immobilized on the electrode surface by a thin layer of PAH assembled on carboxylated carbon nanotubes (CNTs). PAH is cationic polymer acting as bi-functional agent to tightly attach CNTs to the electrode surface and anti-NS1 antibodies through their Fc terminal, avoiding random immobilization. Electrochemical responses of immunoassay are generated at a controlled potential by a reaction between H2O2 and peroxidase enzyme conjugated to anti-NS1 antibodies. RESULTSThe immunosensor developed exhibited a linear range to NS1 varying between 0.1 mu g mL(-1) and 2.5 mu g mL(-1), with clinical range for early diagnostic of acute dengue and a limit of detection of 0.035 mu g mL(-1) that is much lower than the concentration observed from the first day after the onset of fever up to the 9th day. Serum samples are also tested showing good accuracy and specificity. CONCLUSIONSAn immunosensor for NS1 protein of dengue virus was developed. This versatile and reproducible PAH-sandwich platform can be applied to other immunoassays to give reliable and highly sensitive responses. (c) 2014 Society of Chemical Industry
Subject: Multiwalled Carbon Nanotubes
Antigen-capture Elisa
Hydrogen-peroxide
Immunosensor
Antibodies
Diagnosis
Surfaces
Films
Immobilization
Performance
Country: HOBOKEN
Editor: WILEY-BLACKWELL
Rights: fechado
Identifier DOI: 10.1002/jctb.4305
Address: http://onlinelibrary.wiley.com/doi/10.1002/jctb.4305/abstract
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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