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|Title:||Umbilical cord blood cd34(+) stem cells and other mononuclear cell subtypes processed up to 96 h from collection and stored at room temperature maintain a satisfactory functionality for cell therapy|
Metze, K, Metze, I.G.H.L.
|Abstract:||Background and ObjectivesUmbilical cord blood (UCB) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (MNCs) subtypes were viable and functional until 96h after collection, even stored at room temperature. Now, we analyzed the viability and functionality of the cells before and after cryopreservation. Materials and MethodsTwenty UCB units were analyzed at 24 and 96h after collection, frozen for 6months, thawed and re-evaluated. MNCs were analyzed by flow cytometry, viability by 7-AAD and clonogenic assays (CFU) were performed. ResultsAfter 96h of storage, no substantial loss of MNC was found (median 7320x10(6)x6305x10(6)). Percentage and viability CD34(+) cells, B-cell precursors and mesenchymal stem cells were not affected. However, mature B and T lymphocytes as well as granulocytes had a substantial loss. CFU growth was observed in all samples. Prefreezing storage of 96h was associated with a relative loss of colony formation (median 12%). Postthaw, this loss had a median of 49% (24h samples) to 56% (96h samples). ConclusionThe delay of 96h before UCB processing is possible, without a prohibitive impairment of CD34(+) loss in number and functionality.|
Umbilical cord blood (UCB) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (MNCs) subtypes were viable and functional until 96h after collection, even stored at room temperature. Now, we analyzed the
|Citation:||Umbilical Cord Blood Cd34(+) Stem Cells And Other Mononuclear Cell Subtypes Processed Up To 96 H From Collection And Stored At Room Temperature Maintain A Satisfactory Functionality For Cell Therapy. Wiley-blackwell, v. 108, p. 72-81 JAN-2015.|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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