Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/24119
Type: Artigo
Title: Isquemia e reperfusão hepática total associada ao estado de choque hemorrágico controlado: efeitos no seqüestro de neutrófilos no fígado do rato
Title Alternative: Total hepatic warm ischemia and reperfusion associated with controlled hemorrhagic shock: effects of neutrophil sequestration in rat liver
Author: Mantovani, Mario
Hirano, Élcio Shiyoiti
Fontelles, Mauro José
Schenka, André Almeida
Morandin, Rosana Celestina
Abstract: BACKGROUND:The purpose of this experimental study was to evaluate the effects of total hepatic ischemia and reperfusion on the accumulation of neutrophils in the liver of rats, under normal conditions and in rats submitted to controlled hemorrhagic shock . METHODS: Thirty two adult male Wistar rats, were divided into four groups: the Control group, was submitted to the standard procedures for a period of 60 min of observation; Shock group, was submitted to controlled hemorrhagic shock (mean arterial blood pressure = 40 mmHg, 20 min) followed by volume resuscitation (lactated Ringer's solution + blood, 3:1) and reperfusion for 60 min; Pringle group, was submitted to total hepatic ischemia for 15 min and reperfusion for 60 min; The Total group, was submitted to controlled hemorrhagic shock for 15 min followed by volume resuscitation (lactated Ringer's solution + blood, 3:1), total hepatic ischemia for 15 min and reperfusion for 60 min. Measurements of serum lactate and base excess were used to characterize the hemorrhagic shock state with low tissue perfusion. The counting of neutrophils on the liver tissue was performed after the euthanasia of animals. RESULTS: Values for the counting of neutrophils on the liver indicate that, the animals from Pringle group differed from Shock and Total groups (Control 10.30±3.20, Shock 13.94±2.84, Pringle 7.00±3.40, Total 12.45±3.65) but did not differ from Control group. CONCLUSIONS: Rats submitted to controlled hemorrhagic shock state associated to total hepatic ischemia for 15 minutes, followed by 60 minutes of reperfusion, did not present significant neutrophils accumulation on liver tissue.
O propósito deste trabalho experimental foi estudar os efeitos da isquemia e reperfusão hepática total sobre o acúmulo de neutrófilos no fígado de ratos, em condições de normalidade e submetidos ao estado de choque hemorrágico controlado. MÉTODO: Trinta e dois ratos Wistar, machos, foram divididos em quatro grupos de oito animais cada: grupo Controle, submetido à laparotomia com um período de 60 minutos de observação; grupo Choque, submetido a choque hemorrágico controlado (PAM = 40 mmHg, 20 min.) seguido de reposição volêmica (Ringer lactato + sangue, 3:1) e reperfusão (60 min.); grupo Pringle, submetido a isquemia hepática total (15 min.) e reperfusão (60 min.); grupo Total submetido a choque hemorrágico controlado (15 min.) seguido de reposição volêmica (Ringer lactato + sangue, 3:1) mais isquemia hepática total (15 min.) e reperfusão (60 min.). A dosagem do lactato arterial e déficit de base foram utilizados para caracterizar o estado de choque hemorrágico com baixa perfusão tecidual. Após a morte dos animais, procedeu-se à contagem de neutrófilos no tecido hepático. RESULTADOS: Na contagem de neutrófilos no fígado o grupo Pringle diferiu dos grupos Choque e Total, os quais não diferiram entre si (Controle 10,30±3,20; Choque 13,94±2,84; Pringle 7,00±3,40; Total 12,45±3,65). CONCLUSÃO: Em ratos submetidos a estado de choque hemorrágico controlado, associado à isquemia hepática total de 15 minutos, seguido de 60 minutos de reperfusão, não ocorreu acúmulo significativo de neutrófilos no fígado
metadata.dc.description.abstractalternative: The purpose of this experimental study was to evaluate the effects of total hepatic ischemia and reperfusion on the accumulation of neutrophils in the liver of rats, under normal conditions and in rats submitted to controlled hemorrhagic shock . METHODS: Thirty two adult male Wistar rats, were divided into four groups: the Control group, was submitted to the standard procedures for a period of 60 min of observation; Shock group, was submitted to controlled hemorrhagic shock (mean arterial blood pressure = 40 mmHg, 20 min) followed by volume resuscitation (lactated Ringer's solution + blood, 3:1) and reperfusion for 60 min; Pringle group, was submitted to total hepatic ischemia for 15 min and reperfusion for 60 min; The Total group, was submitted to controlled hemorrhagic shock for 15 min followed by volume resuscitation (lactated Ringer's solution + blood, 3:1), total hepatic ischemia for 15 min and reperfusion for 60 min. Measurements of serum lactate and base excess were used to characterize the hemorrhagic shock state with low tissue perfusion. The counting of neutrophils on the liver tissue was performed after the euthanasia of animals. RESULTS: Values for the counting of neutrophils on the liver indicate that, the animals from Pringle group differed from Shock and Total groups (Control 10.30±3.20, Shock 13.94±2.84, Pringle 7.00±3.40, Total 12.45±3.65) but did not differ from Control group. CONCLUSIONS: Rats submitted to controlled hemorrhagic shock state associated to total hepatic ischemia for 15 minutes, followed by 60 minutes of reperfusion, did not present significant neutrophils accumulation on liver tissue
Subject: Choque hemorrágico
Fígado
Isquemia
Neutrófilos
Reperfusão
Country: Brasil
Editor: Colégio Brasileiro de Cirurgiões
Citation: Revista do Colégio Brasileiro de Cirurgiões. Colégio Brasileiro de Cirurgiões, v. 30, n. 4, p. 275-281, 2003.
Rights: aberto
Identifier DOI: 10.1590/S0100-69912003000400005
Address: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912003000400005
Date Issue: 2003
Appears in Collections:FCM - Artigos e Outros Documentos

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