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Type: Artigo de periódico
Title: Validação de metodologia analítica por cromatografia líquida de alta eficiência para quantificação de bupivacaína (S75-R25) em nanoesferas de poli(lactídeo-co-glicolídeo)
Title Alternative: Validation of analytical methodology by HPLC for quantification of bupivacaine (S75-R25) in poli-lactide-co-glicolide nanospheres
Author: Moraes, Carolina Morales
Paula, Eneida de
Rosa, André Henrique
Fraceto, Leonardo Fernandes
Abstract: Bupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile phase and detection at 220 nm. The results obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 10-220.0 g/mL of NovaBupi®. The method was applied to determine the encapsulation efficiency and evaluate the release profile of NovaBupi®, showing good results.
Subject: bupivacaine
PLGA nanospheres
Editor: Sociedade Brasileira de Química
Rights: aberto
Identifier DOI: 10.1590/S0100-40422008000800040
Date Issue: 1-Jan-2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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