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Type: Artigo de periódico
Title: Otimização de método para determinação de flavonóis e flavonas em frutas por cromatografia líquida de alta eficiência utilizando delineamento estatístico e análise de superfície de resposta
Title Alternative: Optimization of a method for determination of flavonols and flavones in fruits by HPLC using statistical design and response surface analysis
Author: Hoffmann-Ribani, Rosemary
Rodriguez-Amaya, Delia B.
Abstract: This work optimized the HPLC conditions for the simultaneous determination of luteolin, apigenin, myricetin, quercetin and kaempferol in aglycone form, as well defined the best conditions for hydrolysis/extraction of these flavonoids in fruits, using the statistical central composite design and response surface analysis. A reverse phase method was developed using a gradient of methanol/water acidified with 0.3% formic acid as mobile phase and a photodiode array detector. The samples were extracted with methanol/water (50:50 v/v) at 90 ºC. The optimum time and HCl concentration varied for the different fruits investigated, demonstrating the necessity of optimizing these conditions for each fruit analyzed. Good recovery (87.1 to 96.3%), repeatability and linearity were obtained.
Subject: flavonoids
HPLC analysis
statistical central composite design
Editor: Sociedade Brasileira de Química
Rights: aberto
Identifier DOI: 10.1590/S0100-40422008000600020
Date Issue: 1-Jan-2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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