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Type: Artigo de periódico
Title: A New Stationary Phase For Analysis Of Hydrophobic Compounds By Rp-lc
Abstract: Hydrophobic selectivity is a key factor that has made C18-bonded phases the main separation materials for HPLC since its development. However, hydrophobic compounds tend to be strongly retained in these phases meaning that large amounts of organic mobile phases are required. In this work, a new stationary phase was prepared from the immobilization of poly(dimethyl-co-alkylmethylsiloxane) (PDAS) over silica particles using microwave radiation. The optimized conditions for preparation of the stationary phase used 720 W of radiation for 10 min. The chromatographic performance and methylene selectivity in the separation of hydrophobic compounds by this new Si(PDAS) phase were similar to those obtained by typical C18-bonded phases. However, the lower hydrophobicity of the organic layer in the Si(PDAS) phase resulted in separation about 40 % faster than typical C18 phases, requiring more aqueous mobile phases. The Si(PDAS) phase showed lower silanophilic activity than that of typical C18 phases due to a more effective polymeric coating of the silica surface. © 2015 Springer-Verlag Berlin Heidelberg
Editor: Friedr. Vieweg und Sohn Verlags GmbH
Citation: Chromatographia. Friedr. Vieweg Und Sohn Verlags Gmbh, v. 79, n. 1, p. 19 - 27, 2015.
Rights: fechado
Identifier DOI: 10.1007/s10337-015-2993-9
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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