Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/23681
Type: Artigo de periódico
Title: Potencial de biocatálise enantiosseletiva de lipases microbianas
Title Alternative: Potential of enantioselective biocatalysis by microbial lipases
Author: Carvalho, Patrícia de O.
Calafatti, Silvana Ap.
Marassi, Maurício
Silva, Daniela M. da
Contesini, Fabiano J.
Bizaco, Renato
Macedo, Gabriela Alves
Abstract: Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed.
Subject: lipases
enantioselectivity
(R,S)-ibuprofen
Editor: Sociedade Brasileira de Química
Rights: aberto
Identifier DOI: 10.1590/S0100-40422005000400012
Address: http://dx.doi.org/10.1590/S0100-40422005000400012
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012
Date Issue: 1-Aug-2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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