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Type: Artigo de periódico
Title: Tissue Microarray Use For Immunohistochemical Study Of Ameloblastoma.
Author: Neves-Silva, Rodrigo
Fonseca, Felipe Paiva
de Jesus, Adriana Souza
Pontes, Hélder Antônio Rebelo
Rocha, André Caroli
Brandão, Thais Bianca
Vargas, Pablo Agustin
Lopes, Márcio Ajudarte
de Almeida, Oslei Paes
Santos-Silva, Alan Roger
Abstract: Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
Subject: Bcl-2
Tissue Microarray
Rights: embargo
Identifier DOI: 10.1111/jop.12428
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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