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Type: Artigo de periódico
Title: A Novel Human Leiomyoma Tissue Derived Matrix For Cell Culture Studies.
Author: Salo, Tuula
Sutinen, Meeri
Hoque Apu, Ehsanul
Sundquist, Elias
Cervigne, Nilva K
de Oliveira, Carine Ervolino
Akram, Saad Ullah
Ohlmeier, Steffen
Suomi, Fumi
Eklund, Lauri
Juusela, Pirjo
Åström, Pirjo
Bitu, Carolina Cavalcante
Santala, Markku
Savolainen, Kalle
Korvala, Johanna
Paes Leme, Adriana Franco
Coletta, Ricardo D
Abstract: The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel. A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. We demonstrated that only 34 % of Myogel's molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® in in vitro Transwell® invasion and capillary formation assays. In conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers.
Citation: Bmc Cancer. v. 15, n. 1, p. 981, 2015.
Rights: aberto
Identifier DOI: 10.1186/s12885-015-1944-z
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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