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Type: Artigo de periódico
Title: Melatonin Attenuates The Tlr4-mediated Inflammatory Response Through Myd88- And Trif-dependent Signaling Pathways In An In Vivo Model Of Ovarian Cancer.
Author: Chuffa, Luiz Gustavo A
Fioruci-Fontanelli, Beatriz A
Mendes, Leonardo O
Ferreira Seiva, Fábio R
Martinez, Marcelo
Fávaro, Wagner J
Domeniconi, Raquel F
Pinheiro, Patrícia F F
Delazari Dos Santos, Lucilene
Martinez, Francisco Eduardo
Abstract: Toll-like receptors (TLRs) are effector molecules expressed on the surface of ovarian cancer (OC) cells, but the functions of the TLR2/TLR4 signaling pathways in these cells remain unclear. Melatonin (mel) acts as an anti-inflammatory factor and has been reported to modulate TLRs in some aggressive tumor cell types. Therefore, we investigated OC and the effect of long-term mel therapy on the signaling pathways mediated by TLR2 and TLR4 via myeloid differentiation factor 88 (MyD88) and toll-like receptor-associated activator of interferon (TRIF) in an ethanol-preferring rat model. To induce OC, the left ovary of animals either consuming 10% (v/v) ethanol or not was injected directly under the bursa with a single dose of 100 μg of 7,12-dimethylbenz(a)anthracene (DMBA) dissolved in 10 μL of sesame oil. The right ovaries were used as sham-surgery controls. After developing OC, half of the animals received i.p. injections of mel (200 μg/100 g b.w./day) for 60 days. Although mel therapy was unable to reduce TLR2 levels, it was able to suppress the OC-associated increase in the levels of the following proteins: TLR4, MyD88, nuclear factor kappa B (NFkB p65), inhibitor of NFkB alpha (IkBα), IkB kinase alpha (IKK-α), TNF receptor-associated factor 6 (TRAF6), TRIF, interferon regulatory factor 3 (IRF3), interferon β (IFN-β), tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. In addition, mel significantly attenuated the expression of IkBα, NFkB p65, TRIF and IRF-3, which are involved in TLR4-mediated signaling in OC during ethanol intake. Collectively, our results suggest that mel attenuates the TLR4-induced MyD88- and TRIF-dependent signaling pathways in ethanol-preferring rats with OC.
Subject: Adaptor Proteins, Vesicular Transport
Disease Models, Animal
Gene Expression Regulation, Neoplastic
Inflammation Mediators
Myeloid Differentiation Factor 88
Nf-kappa B
Ovarian Neoplasms
Signal Transduction
Toll-like Receptor 2
Toll-like Receptor 4
Citation: Bmc Cancer. v. 15, p. 34, 2015.
Rights: aberto
Identifier DOI: 10.1186/s12885-015-1032-4
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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