Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235989
Type: Artigo de periódico
Title: Revealing Praziquantel Molecular Targets Using Mass Spectrometry Imaging: An Expeditious Approach Applied To Schistosoma Mansoni.
Author: Ferreira, Mônica Siqueira
de Oliveira, Rosimeire Nunes
de Oliveira, Diogo Noin
Esteves, Cibele Zanardi
Allegretti, Silmara Marques
Catharino, Rodrigo Ramos
Abstract: Finding specific molecular targets and the mechanism of action of praziquantel in the treatment of schistosomiasis remains a challenging task. Our efforts were focused on obtaining further information on worm composition before and after exposure to praziquantel in the treatment of schistosomiasis to elucidate the potential sites of action of this drug. Evidence indicates that the lipid bilayer is changed by treatment with praziquantel. Following this rationale, we employed a mass spectrometry imaging-based approach that helped to characterise lipids in specific locations, which are directly involved in the biochemical pathways of the BH strain of Schistosoma mansoni, as well as differentiating the molecular response that each worm sex presents in vivo. Our findings demonstrated significant differences between the chemical markers found in adult worms before and after praziquantel exposure, especially in phospholipids, which were predominantly identified as chemical markers in all samples. Results also indicate that distinct molecular pathways in both male and female worms could be differentially affected by praziquantel treatment. These data shine new light on the mechanism of action of praziquantel, taking a further step towards its full understanding.
Subject: Biomarkers
Lipids
Mass Spectrometry Imaging
Praziquantel
Schistosoma Mansoni
Citation: International Journal For Parasitology. v. 45, n. 6, p. 385-391, 2015-May.
Rights: embargo
Identifier DOI: 10.1016/j.ijpara.2014.12.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/25812833
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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