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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAnalysis Of Genomic Regions Of Trichoderma Harzianum Ioc-3844 Related To Biomass Degradation.pt_BR
dc.contributor.authorCrucello, Alinept_BR
dc.contributor.authorSforça, Danilo Augustopt_BR
dc.contributor.authorHorta, Maria Augusta Crivelentept_BR
dc.contributor.authordos Santos, Clelton Aparecidopt_BR
dc.contributor.authorViana, Américo José Carvalhopt_BR
dc.contributor.authorBeloti, Lilian Luziapt_BR
dc.contributor.authorde Toledo, Marcelo Augusto Szymanskipt_BR
dc.contributor.authorVincentz, Michelpt_BR
dc.contributor.authorKuroshu, Reginaldo Massanobupt_BR
dc.contributor.authorde Souza, Anete Pereirapt_BR
unicamp.authorAline Crucello, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorDanilo Augusto Sforça, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorMaria Augusta Crivelente Horta, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorClelton Aparecido dos Santos, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorAmérico José Carvalho Viana, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorLilian Luzia Beloti, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorMarcelo Augusto Szymanski de Toledo, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorMichel Vincentz, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazilpt_BR
unicamp.authorInstituto de Biologia (IB), Universidade Estadual de Campinas, Departamento de Biologia Vegetal, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorAnete Pereira de Souza, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, Campinas, São Paulo, Brazilpt_BR
unicamp.authorInstituto de Biologia (IB), Universidade Estadual de Campinas, Departamento de Biologia Vegetal, Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalReginaldo Massanobu Kuroshu, Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos, São Paulo, Brazil.pt
dc.description.abstractTrichoderma harzianum IOC-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications in second-generation bioethanol production. However, the T. harzianum biomass degradation mechanism has not been well explored at the genetic level. The present work investigates six genomic regions (~150 kbp each) in this fungus that are enriched with genes related to biomass conversion. A BAC library consisting of 5,760 clones was constructed, with an average insert length of 90 kbp. The assembled BAC sequences revealed 232 predicted genes, 31.5% of which were related to catabolic pathways, including those involved in biomass degradation. An expression profile analysis based on RNA-Seq data demonstrated that putative regulatory elements, such as membrane transport proteins and transcription factors, are located in the same genomic regions as genes related to carbohydrate metabolism and exhibit similar expression profiles. Thus, we demonstrate a rapid and efficient tool that focuses on specific genomic regions by combining a BAC library with transcriptomic data. This is the first BAC-based structural genomic study of the cellulolytic fungus T. harzianum, and its findings provide new perspectives regarding the use of this species in biomass degradation processes.en
dc.relation.ispartofPlos Onept_BR
dc.relation.ispartofabbreviationPLoS ONEpt_BR
dc.date.issued2015pt_BR
dc.identifier.citationPlos One. v. 10, n. 4, p. e0122122, 2015.pt_BR
dc.language.isoengpt_BR
dc.description.volume10pt_BR
dc.description.firstpagee0122122pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.doi10.1371/journal.pone.0122122pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25836973pt_BR
dc.date.available2016-05-23T19:43:21Z-
dc.date.accessioned2016-05-23T19:43:21Z-
dc.description.provenanceMade available in DSpace on 2016-05-23T19:43:21Z (GMT). No. of bitstreams: 1 pmed_25836973.pdf: 2400043 bytes, checksum: 59905b5fae0eb2f20c5d9d9d859bc537 (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235957-
dc.identifier.idPubmed25836973pt_BR
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