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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExogenous Sucrose Supply Changes Sugar Metabolism And Reduces Photosynthesis Of Sugarcane Through The Down-regulation Of Rubisco Abundance And Activity.pt_BR
dc.contributor.authorLobo, Ana Karla Moreirapt_BR
dc.contributor.authorde Oliveira Martins, Marciopt_BR
dc.contributor.authorLima Neto, Milton Costapt_BR
dc.contributor.authorMachado, Eduardo Carusopt_BR
dc.contributor.authorRibeiro, Rafael Vasconcelospt_BR
dc.contributor.authorSilveira, Joaquim Albenisio Gomespt_BR
unicamp.authorRafael Vasconcelos Ribeiro, Department of Plant Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, 255, CEP 13083-862 Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalAna Karla Moreira Lobo, Laboratório de Metabolismo de Plantas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, S/N, CP 6004, CEP 60440-970 Fortaleza, Ceará, Brazil.pt
unicamp.author.externalMarcio de Oliveira Martins, Laboratório de Metabolismo de Plantas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, S/N, CP 6004, CEP 60440-970 Fortaleza, Ceará, Brazil.pt
unicamp.author.externalMilton Costa Lima Neto, Laboratório de Metabolismo de Plantas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, S/N, CP 6004, CEP 60440-970 Fortaleza, Ceará, Brazil.pt
unicamp.author.externalEduardo Caruso Machado, Laboratório de Fisiologia Vegetal Coaracy M. Franco, Centro de Pesquisa e Desenvolvimento de Ecofisiologia e Biofísica, Instituto Agronômico (IAC), Avenida Barão de Itapura, 1481, CP 28, CEP 13012-970 Campinas, São Paulo, Brazil.pt
unicamp.author.externalJoaquim Albenisio Gomes Silveira, Laboratório de Metabolismo de Plantas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, S/N, CP 6004, CEP 60440-970 Fortaleza, Ceará, Brazil. Electronic address: silveira@ufc.br.pt
dc.subjectCo(2) Assimilationpt_BR
dc.subjectPepcase Activitypt_BR
dc.subjectPhotosynthetic Modulationpt_BR
dc.subjectSaccharum Spp.pt_BR
dc.subjectSource-sinkpt_BR
dc.description.abstractPhotosynthetic modulation by sugars has been known for many years, but the biochemical and molecular comprehension of this process is lacking. We studied how the exogenous sucrose supplied to leaves could affect sugar metabolism in leaf, sheath and stalk and inhibit photosynthesis in four-month old sugarcane plants. Exogenous sucrose 50mM sprayed on attached leaves strongly impaired the net CO2 assimilation (PN) and decreased the instantaneous carboxylation efficiency (PN/Ci), suggesting that the impairment in photosynthesis was caused by biochemical restrictions. The photosystem II activity was also affected by excess sucrose as indicated by the reduction in the apparent electron transport rate, effective quantum yield and increase in non-photochemical quenching. In leaf segments, sucrose accumulation was related to increases in the activities of soluble acid and neutral invertases, sucrose synthase and sucrose phosphate synthase, whereas the contents of fructose increased and glucose slightly decreased. Changes in the activities of sucrose hydrolyzing and synthesizing enzymes in leaf, sheath and stalk and sugar profile in intact plants were not enough to identify which sugar(s) or enzyme(s) were directly involved in photosynthesis modulation. However, exogenous sucrose was able to trigger down-regulation in the Rubisco abundance, activation state and enzymatic activity. Despite the fact that PN/Ci had been notably decreased by sucrose, in vitro activity and abundance of PEPCase did not change, suggesting an in vivo modulation of this enzyme. The data reveal that sucrose and/or other derivative sugars in leaves inhibited sugarcane photosynthesis by down-regulation of Rubisco synthesis and activity. Our data also suggest that sugar modulation was not exerted by a feedback mechanism induced by the accumulation of sugars in immature sugarcane stalk.en
dc.relation.ispartofJournal Of Plant Physiologypt_BR
dc.relation.ispartofabbreviationJ. Plant Physiol.pt_BR
dc.date.issued2015-Maypt_BR
dc.identifier.citationJournal Of Plant Physiology. v. 179, p. 113-121, 2015-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume179pt_BR
dc.description.firstpage113-121pt_BR
dc.rightsembargopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1618-1328pt_BR
dc.identifier.doi10.1016/j.jplph.2015.03.007pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25863283pt_BR
dc.date.available2016-05-23T19:43:15Z-
dc.date.accessioned2016-05-23T19:43:15Z-
dc.description.provenanceMade available in DSpace on 2016-05-23T19:43:15Z (GMT). No. of bitstreams: 1 pmed_25863283.pdf: 1113244 bytes, checksum: 49c1458d8aca1b5e9d2782511c0bc2d2 (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235932-
dc.identifier.idPubmed25863283pt_BR
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