Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/23587
Type: Artigo de periódico
Title: Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
Title Alternative: Effect of poly (ethylene) glycol and albumin on the immobilization of microbial lipase and catalysis and catalysis in organic media
Author: Soares, Cleide Mara Faria
Santana, Maria Helena Andrade
Zanin, Gisella M.
Castro, Heizir F. de
Abstract: Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.
Subject: additives
controlled pore silica
immobilized lipase
Editor: Sociedade Brasileira de Química
Rights: aberto
Identifier DOI: 10.1590/S0100-40422003000600010
Address: http://dx.doi.org/10.1590/S0100-40422003000600010
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422003000600010
Date Issue: 1-Dec-2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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