Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235722
Type: Artigo de periódico
Title: Vapd In Xylella Fastidiosa Is A Thermostable Protein With Ribonuclease Activity.
Author: Mendes, Juliano S
Santiago, André da S
Toledo, Marcelo A S
Rosselli-Murai, Luciana K
Favaro, Marianna T P
Santos, Clelton A
Horta, Maria Augusta C
Crucello, Aline
Beloti, Lilian L
Romero, Fabian
Tasic, Ljubica
de Souza, Alessandra A
de Souza, Anete P
Abstract: Xylella fastidiosa strain 9a5c is a gram-negative phytopathogen that is the causal agent of citrus variegated chlorosis (CVC), a disease that is responsible for economic losses in Brazilian agriculture. The most well-known mechanism of pathogenicity for this bacterial pathogen is xylem vessel occlusion, which results from bacterial movement and the formation of biofilms. The molecular mechanisms underlying the virulence caused by biofilm formation are unknown. Here, we provide evidence showing that virulence-associated protein D in X. fastidiosa (Xf-VapD) is a thermostable protein with ribonuclease activity. Moreover, protein expression analyses in two X. fastidiosa strains, including virulent (Xf9a5c) and nonpathogenic (XfJ1a12) strains, showed that Xf-VapD was expressed during all phases of development in both strains and that increased expression was observed in Xf9a5c during biofilm growth. This study is an important step toward characterizing and improving our understanding of the biological significance of Xf-VapD and its potential functions in the CVC pathosystem.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0145765
Address: http://www.ncbi.nlm.nih.gov/pubmed/26694028
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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