Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235563
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDifferential Profile Of Pip4k2a Expression In Hematological Malignancies.pt_BR
dc.contributor.authorLima, Kelipt_BR
dc.contributor.authorRibeiro, Daniela Mariapt_BR
dc.contributor.authorCampos, Paula de Melopt_BR
dc.contributor.authorCosta, Fernando Ferreirapt_BR
dc.contributor.authorTraina, Fabiolapt_BR
dc.contributor.authorSaad, Sara Teresinha Olallapt_BR
dc.contributor.authorSonati, Maria de Fátimapt_BR
dc.contributor.authorMachado-Neto, João Agostinhopt_BR
unicamp.authorKeli Lima, Department of Clinical Pathology, School of Medical Sciences, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorDaniela Maria Ribeiro, Department of Clinical Pathology, School of Medical Sciences, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorPaula de Melo Campos, Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorFernando Ferreira Costa, Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorFabiola Traina, Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorSara Teresinha Olalla Saad, Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorMaria de Fátima Sonati, Department of Clinical Pathology, School of Medical Sciences, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorJoão Agostinho Machado-Neto, Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil. Electronic address: jamachadoneto@gmail.com.pt_BR
dc.subjectAlphapt_BR
dc.subjectCell Viabilitypt_BR
dc.subjectLeukemiapt_BR
dc.subjectMyelodysplastic Syndromespt_BR
dc.subjectPip4k2apt_BR
dc.subjectPhosphatidylinositolpt_BR
dc.subjectPhosphatidylinositol-5-phosphate 4-kinasept_BR
dc.subjectType Iipt_BR
dc.description.abstractPIP4K2A is a lipid kinase that phosphorylates PtdIns5P, generating PtdIns4,5P2. Recently, PIP4K2A was identified as a potential target in acute myeloid leukemia cells. The objective of the present study was to investigate the PIP4K2A expression in hematological malignancies and verify the effects of PIP4K2A silencing on proliferation and survival of leukemia cell lines. PIP4K2A was found to be a cytoplasmic and nuclear protein with reduced levels in leukemia cell lines compared to normal leukocytes. PIP4K2A mRNA levels were significantly reduced in bone marrow cells from acute lymphoid leukemia (ALL) patients compared with healthy donors and in myelodysplastic syndromes (MDS) with ≥5% compared with <5% bone marrow blasts. Low PIP4K2A expression (lowest tertile versus 2 higher tertiles) negatively impacted overall survival of MDS patients by univariate analysis. PIP4K2A silencing did not modulate cell proliferation, clonogenicity and apoptosis of HEL and Namalwa leukemia cells. In summary, we characterized the expression of PIP4K2A in a cohort of patients with hematological malignancies and we found that PIP4K2A mRNA expression is downregulated in RAEB-1/RAEB-2 MDS and ALL cells, and PIP4K2A silencing does not modulate cell survival in HEL and Namalwa leukemia cells.en
dc.relation.ispartofBlood Cells, Molecules & Diseasespt_BR
dc.relation.ispartofabbreviationBlood Cells Mol. Dis.pt_BR
dc.date.issued2015-Octpt_BR
dc.identifier.citationBlood Cells, Molecules & Diseases. v. 55, n. 3, p. 228-235, 2015-Oct.pt_BR
dc.language.isoengpt_BR
dc.description.volume55pt_BR
dc.description.firstpage228-235pt_BR
dc.rightsembargopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1096-0961pt_BR
dc.identifier.doi10.1016/j.bcmd.2015.06.014pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/?term=26227852pt_BR
dc.date.available2016-05-23T19:41:31Z-
dc.date.accessioned2016-05-23T19:41:31Z-
dc.description.provenanceMade available in DSpace on 2016-05-23T19:41:31Z (GMT). No. of bitstreams: 1 pmed_26227852.pdf: 854371 bytes, checksum: 2c55d464225deb9900265fbbafaaa6ab (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235563-
dc.identifier.idPubmed26227852pt_BR
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