Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235445
Type: Artigo de periódico
Title: Heat Treatment Of Samples Improve The Performance Of The Nijmegen-bethesda Assay In Hemophilia A Patients Undergoing Immune Tolerance Induction.
Author: de Lima Montalvão, Silmara Aparecida
Tucunduva, Alini Camargo
de Almeida Sambo, Andrea Luísa
De Paula, Erich Vinicius
de Souza Medina, Samuel
Ozelo, Margareth Castro
Abstract: Nijmegen-Bethesda assay is the gold standard to assess inhibitory antibodies against factor (F) VIII. This method has some limitations, including high coefficient of variation and possible interference of residual endogenous or exogenous factor VIII. Heat-treatment of samples at 56°C for 30min could be a strategy to improve the sensitivity of this test. The aim of this study was to compare inhibitor quantification in hemophilia patients with and without inhibitor performed in previously heated and non-heated samples. A total of 109 analyses from 46 patients with severe hemophilia A were performed. Patients were divided into three groups: 20 patients with no history of inhibitor, recently and not recently exposed to FVIII (group I), 21 patients with history of inhibitor not exposed to FVIII (group II), and 5 patients (68 samples) undergoing an immune tolerance induction (ITI) protocol (group III). For patients with no history of inhibitor, heat-treatment did not modify the results (p=0.24). However, differences in inhibitor levels between heated and non-heated samples were observed in patients with history of inhibitor (group II, p<0.05) and in patients in ITI (group III, p<0.001). In 11 samples, inhibitor quantification shifted from negative to positive. Additionally, a longitudinal evaluation of each ITI patient showed similar trend line for the results of heated and non-heated samples. In this study, we demonstrated that heating samples increase sensitivity of Nijmegen-Bethesda assay, with no shift from negative to positive results in patients with no history of inhibitor. Furthermore, this procedure has an important role to patients undergoing an ITI protocol.
Subject: Hemophilia
Immune Tolerance Induction
Inhibitor
Nijmegen–bethesda Assay
Citation: Thrombosis Research. v. 136, n. 6, p. 1280-1284, 2015-Dec.
Rights: embargo
Identifier DOI: 10.1016/j.thromres.2015.08.014
Address: http://www.ncbi.nlm.nih.gov/pubmed/?term=26344704
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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