Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235305
Type: Artigo de periódico
Title: Changes In Calsequestrin, Tnf-α, Tgf-β And Myod Levels During The Progression Of Skeletal Muscle Dystrophy In Mdx Mice: A Comparative Analysis Of The Quadriceps, Diaphragm And Intrinsic Laryngeal Muscles.
Author: Barros Maranhão, Juliana
de Oliveira Moreira, Drielen
Maurício, Adriana Fogagnolo
de Carvalho, Samara Camaçari
Ferretti, Renato
Pereira, Juliano Alves
Santo Neto, Humberto
Marques, Maria Julia
Abstract: In Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-α; pro-inflammatory cytokine), tumour growth factor (TGF-β; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-α, TGF-β and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-α and TGF-β serve as markers of dystrophy primarily for the diaphragm.
Subject: Calsequestrin
Dystrophy
Mdx
Tumour Growth Factor-β
Tumour Necrosis Factor-α
Citation: International Journal Of Experimental Pathology. v. 96, n. 5, p. 285-293, 2015-Oct.
Rights: embargo
Identifier DOI: 10.1111/iep.12142
Address: http://www.ncbi.nlm.nih.gov/pubmed/26515458
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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