Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235237
Type: Artigo de periódico
Title: Actinobacteria From Termite Mounds Show Antiviral Activity Against Bovine Viral Diarrhea Virus, A Surrogate Model For Hepatitis C Virus.
Author: Padilla, Marina Aiello
Rodrigues, Rodney Alexandre Ferreira
Bastos, Juliana Cristina Santiago
Martini, Matheus Cavalheiro
Barnabé, Ana Caroline de Souza
Kohn, Luciana Konecny
Uetanabaro, Ana Paula Trovatti
Bomfim, Getúlio Freitas
Afonso, Rafael Sanches
Fantinatti-Garboggini, Fabiana
Arns, Clarice Weis
Abstract: Extracts from termite-associated bacteria were evaluated for in vitro antiviral activity against bovine viral diarrhea virus (BVDV). Two bacterial strains were identified as active, with percentages of inhibition (IP) equal to 98%. Both strains were subjected to functional analysis via the addition of virus and extract at different time points in cell culture; the results showed that they were effective as posttreatments. Moreover, we performed MTT colorimetric assays to identify the CC50, IC50, and SI values of these strains, and strain CDPA27 was considered the most promising. In parallel, the isolates were identified as Streptomyces through 16S rRNA gene sequencing analysis. Specifically, CDPA27 was identified as S. chartreusis. The CDPA27 extract was fractionated on a C18-E SPE cartridge, and the fractions were reevaluated. A 100% methanol fraction was identified to contain the compound(s) responsible for antiviral activity, which had an SI of 262.41. GC-MS analysis showed that this activity was likely associated with the compound(s) that had a peak retention time of 5 min. Taken together, the results of the present study provide new information for antiviral research using natural sources, demonstrate the antiviral potential of Streptomyces chartreusis compounds isolated from termite mounds against BVDV, and lay the foundation for further studies on the treatment of HCV infection.
Rights: aberto
Identifier DOI: 10.1155/2015/745754
Address: http://www.ncbi.nlm.nih.gov/pubmed/26579205
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_26579205.pdf750.89 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.