Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/235233
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIncreased Susceptibility Of Gracilinanus Microtarsus Liver Mitochondria To Ca²⁺-induced Permeability Transition Is Associated With A More Oxidized State Of Nad(p).pt_BR
dc.contributor.authorRonchi, Juliana Apt_BR
dc.contributor.authorHenning, Barbarapt_BR
dc.contributor.authorRavagnani, Felipe Gpt_BR
dc.contributor.authorFigueira, Tiago Rpt_BR
dc.contributor.authorCastilho, Roger Fpt_BR
dc.contributor.authordos Reis, Sergio Fpt_BR
dc.contributor.authorVercesi, Anibal Ept_BR
unicamp.authorJuliana A Ronchi, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorBarbara Henning, Graduate Program in Ecology, Biology Institute, State University of Campinas, 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorFelipe G Ravagnani, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorTiago R Figueira, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorRoger F Castilho, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorSergio F dos Reis, Department of Animal Biology, Biology Institute, State University of Campinas, 13087-877 Campinas, SP, Brazil.pt_BR
unicamp.authorAnibal E Vercesi, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13087-877 Campinas, SP, Brazil.pt_BR
dc.description.abstractIn addition to be the cell's powerhouse, mitochondria also contain a cell death machinery that includes highly regulated processes such as the membrane permeability transition pore (PTP) and reactive oxygen species (ROS) production. In this context, the results presented here provide evidence that liver mitochondria isolated from Gracilinanus microtarsus, a small and short life span (one year) marsupial, when compared to mice, are much more susceptible to PTP opening in association with a poor NADPH dependent antioxidant capacity. Liver mitochondria isolated from the marsupial are well coupled and take up Ca(2+) but exhibited a much lower Ca(2+) retention capacity than mouse mitochondria. Although the known PTP inhibitors cyclosporin A, ADP, and ATP significantly increased the marsupial mitochondria capacity to retain Ca(2+), their effects were much larger in mice than in marsupial mitochondria. Both fluorescence and HPLC analysis of mitochondrial nicotinamide nucleotides showed that both content and state of reduction (mainly of NADPH) were lower in the marsupial mitochondria than in mice mitochondria despite the similarity in the activity of the glutathione peroxidase/reductase system. Overall, these data suggest that PTP opening is an important event in processes of Ca(2+) signalling to cell death mediated by mitochondrial redox imbalance in G. microtarsus.en
dc.relation.ispartofOxidative Medicine And Cellular Longevitypt_BR
dc.relation.ispartofabbreviationOxid Med Cell Longevpt_BR
dc.date.issued2015pt_BR
dc.identifier.citationOxidative Medicine And Cellular Longevity. v. 2015, p. 940627, 2015.pt_BR
dc.language.isoengpt_BR
dc.description.volume2015pt_BR
dc.description.firstpage940627pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1942-0994pt_BR
dc.identifier.doi10.1155/2015/940627pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26583063pt_BR
dc.date.available2016-05-23T19:40:13Z-
dc.date.accessioned2016-05-23T19:40:13Z-
dc.description.provenanceMade available in DSpace on 2016-05-23T19:40:13Z (GMT). No. of bitstreams: 1 pmed_26583063.pdf: 959604 bytes, checksum: 564afee3474ab850590a8d3941b837fa (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235233-
dc.identifier.idPubmed26583063pt_BR
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