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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleTranscriptome Analysis In Coffea Eugenioides, An Arabica Coffee Ancestor, Reveals Differentially Expressed Genes In Leaves And Fruits.pt_BR
dc.contributor.authorYuyama, Priscila Marypt_BR
dc.contributor.authorReis Júnior, Osvaldopt_BR
dc.contributor.authorIvamoto, Suzana Tiemipt_BR
dc.contributor.authorDomingues, Douglas Silvapt_BR
dc.contributor.authorCarazzolle, Marcelo Falsarellapt_BR
dc.contributor.authorPereira, Gonçalo Amarante Guimarãespt_BR
dc.contributor.authorCharmetant, Pierrept_BR
dc.contributor.authorLeroy, Thierrypt_BR
dc.contributor.authorPereira, Luiz Filipe Protasiopt_BR
unicamp.authorOsvaldo Reis Júnior, Laboratório de Genômica e Expressão, Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
unicamp.authorMarcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão, Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
unicamp.authorGonçalo Amarante Guimarães Pereira, Laboratório de Genômica e Expressão, Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalPriscila Mary Yuyama, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil. Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brazil.pt
unicamp.author.externalSuzana Tiemi Ivamoto, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil. Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brazil.pt
unicamp.author.externalDouglas Silva Domingues, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brazil. Department of Botany, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista (UNESP), Rio Claro, São Paulo, Brazil.pt
unicamp.author.externalPierre Charmetant, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, 34398, Montpellier, France.pt
unicamp.author.externalThierry Leroy, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, 34398, Montpellier, France.pt
unicamp.author.externalLuiz Filipe Protasio Pereira, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brazil. filipe.pereira@embrapa.br. Embrapa Café, Brasília, DF, Brazil. filipe.pereira@embrapa.br.pt
dc.subjectCoffeapt_BR
dc.subjectDifferentially Expressed Genespt_BR
dc.subjectGene Annotationpt_BR
dc.subjectHomeologouspt_BR
dc.subjectRna-seqpt_BR
dc.description.abstractStudies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next-generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications.en
dc.relation.ispartofMolecular Genetics And Genomics : Mggpt_BR
dc.relation.ispartofabbreviationMol. Genet. Genomicspt_BR
dc.date.issued2016-Febpt_BR
dc.identifier.citationMolecular Genetics And Genomics : Mgg. v. 291, n. 1, p. 323-336, 2016-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume291pt_BR
dc.description.firstpage323-336pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1617-4623pt_BR
dc.identifier.doi10.1007/s00438-015-1111-xpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26334613pt_BR
dc.date.available2016-05-23T19:39:51Z-
dc.date.accessioned2016-05-23T19:39:51Z-
dc.description.provenanceMade available in DSpace on 2016-05-23T19:39:51Z (GMT). No. of bitstreams: 1 pmed_26334613.pdf: 1370292 bytes, checksum: d7fddcebea8be485024cebfee1fe515c (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235142-
dc.identifier.idPubmed26334613pt_BR
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