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Type: Artigo de periódico
Title: Losartan And Captopril Treatment Rescue Normal Thrombus Formation In Microfibril Associated Glycoprotein-1 (magp1) Deficient Mice.
Author: Vassequi-Silva, Tallita
Pereira, Danielle Sousa
Nery Diez, Ana Cláudia C
Braga, Guilherme G
Godoy, Juliana A
Mendes, Camila B
Dos Santos, Leonardo
Krieger, José E
Antunes, Edson
Costa, Fábio T M
Vicente, Cristina P
Werneck, Claudio C
Abstract: MAGP1 is a glycoprotein present in the elastic fibers and is a part of the microfibrils components. MAGP1 interacts with von Willebrand factor and the active form of TGF-β and BMP. In mice lacking MAGP1, thrombus formation is delayed, increasing the occlusion time of carotid artery despite presenting normal blood coagulation in vitro. MAGP1-containing microfibrils may play a role in hemostasis and thrombosis. In this work, we evaluated the function of MAGP1 and its relation to TGF-β in the arterial thrombosis process. We analyzed thrombus formation time in wild type and MAGP1-deficient mice comparing Rose Bengal and Ferric Chloride induced arterial lesion. The potential participation of TGF-β in this process was accessed when we treated both wild type and MAGP1-deficient mice with losartan (an antihypertensive drug that decreases TGF-β activity) or captopril (an angiotensin converting enzyme inhibitor that was used as a control antihypertensive drug). Besides, we evaluated thrombus embolization and the gelatinolytic activity in the arterial walls in vitro and ex vivo. Losartan and captopril were able to recover the thrombus formation time without changing blood pressure, activated partial thromboplastin time (aPTT), PT (prothrombin time), platelet aggregation and adhesion, but decreased gelatinase activity. Our results suggest that both treatments are effective in the prevention of the sub-endothelial ECM degradation, allowing the recovery of normal thrombus formation.
Subject: Antihypertensive Drugs
Arterial Thrombosis
Extracellular Matrix
Matrix Metalloproteinases
Microfibril-associated Glycoprotein
Transforming Growth Factor-β
Rights: embargo
Identifier DOI: 10.1016/j.thromres.2015.12.004
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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