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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleSurvival In Vitro and Virulence of Trypanosoma cruzi in Acai Pulp in Experimental Acute Chagas Diseasept_BR
dc.contributor.authorBarbosa, Rodrigo Labellopt_BR
dc.contributor.authorDias, Viviane Liottipt_BR
dc.contributor.authorPereira, Karen Signoript_BR
dc.contributor.authorSchmidt, Flavio Luispt_BR
dc.contributor.authorBueno Franco, Regina Maurapt_BR
dc.contributor.authorAparecida Guaraldo, Ana Mariapt_BR
dc.contributor.authorAlves, Delma Pegolopt_BR
dc.contributor.authorCorrea Passos, Luiz Augustopt_BR
unicamp.authorBarbosa, Rodrigo Labellopt_BR
unicamp.authorDias, Viviane Liottipt_BR
unicamp.authorSchmidt, Flavio Luispt_BR
unicamp.authorBueno Franco, Regina Maurapt_BR
unicamp.authorAparecida Guaraldo, Ana Mariapt_BR
unicamp.authorAlves, Delma Pegolopt_BR
unicamp.authorCorrea Passos, Luiz Augustopt_BR
dc.subject.wosORAL-TRANSMISSIONpt_BR
dc.subject.wosFOODpt_BR
dc.description.abstractChagas disease is a parasitic infection with high socioeconomic impact throughout Latin America. Although this severe, incurable disease can be transmitted by several routes, oral transmission is currently the most important route in the Amazon Basin. Acai pulp has nutritional properties and is popular throughout Brazil and abroad. However, this pulp has been associated with microepidemics of acute Chagas disease (ACD) in northern Brazil, where agar fruit is the main food supplement. In this study, we examined the in vitro survival and in vivo virulence of Trypanosoma cruzi Y strain in acai pulp. Aliquots of in natura acai pulp produced in Belem city in the northern Brazilian state of Para were mixed with 10(5) trypomastigotes. The samples were incubated at room temperature or at 4 or 20 C for various periods, and the parasites were isolated by forced sieving. The resulting eluates were examined by microscopy, and the trypomastigotes were administered intraperitoneally, orally, or by gavage to immunodeficient mice (C.B-17-Prkdc(scid)/PasUnib) that had been pretreated with antibiotics. Parasitemia was quantified by the Brener method, and mortality was recorded daily. All routes of administration resulted in ACD. A 5-day delay in the onset of parasitemia occurred with oral administration. The survival and virulence of the parasites were unaffected by prior incubation at room temperature for 24 h, at 4 degrees C for 144 h, and at -20 degrees C for 26 h. These results indicate that T. cruzi can survive and retain its virulence in acai pulp under various conditions and that cooling and freezing are not suitable methods for preventing foodborne ACD.pt
dc.relation.ispartofJournal of Food Protectionpt_BR
dc.publisher.cityDes Moinespt_BR
dc.publisherInt Assoc Food Protectionpt_BR
dc.date.issued2012pt_BR
dc.identifier.citationJournal of Food Protection. Int Assoc Food Protection, v.75, n.3, p.601-606, 2012pt_BR
dc.language.isoengpt_BR
dc.description.volume75pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage601pt_BR
dc.description.lastpage606pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0362-028Xpt_BR
dc.identifier.wosidWOS:000301406400027pt_BR
dc.identifier.doi10.4315/0362-028X.JFP-11-233pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipBrazilian Ministry of Healthpt_BR
dc.description.sponsorshipBrazilian Ministry of Health [667/2008]pt_BR
dc.description.sponsorshipUniversidade Estadual de Campinas entitled [667/2008]pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2013-09-19T18:06:31Z
dc.date.available2016-07-01T14:37:40Z-
dc.date.accessioned2013-09-19T18:06:31Z
dc.date.accessioned2016-07-01T14:37:40Z-
dc.description.provenanceMade available in DSpace on 2013-09-19T18:06:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.description.provenanceMade available in DSpace on 2016-07-01T14:37:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2220
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2220-
dc.contributor.departmentBiologia Animal
dc.contributor.unidadeIBpt
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