Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/21648
Type: Artigo de periódico
Title: In vitro susceptibility of Plasmodium falciparum Welch field isolates to infusions prepared from Artemisia annua L. cultivated in the Brazilian Amazon
Author: Silva, Luiz Francisco Rocha e
Magalhães, Pedro Melillo de
Costa, Mônica Regina Farias
Alecrim, Maria das Graças Costa
Chaves, Francisco Célio Maia
Hidalgo, Ari de Freitas
Pohlit, Adrian Martin
Vieira, Pedro Paulo Ribeiro
Abstract: Artemisinin is the active antimalarial compound obtained from the leaves of Artemisia annua L. Artemisinin, and its semi-synthetic derivatives, are the main drugs used to treat multi-drug-resistant Plasmodium falciparum (one of the human malaria parasite species). The in vitro susceptibility of P. falciparum K1 and 3d7 strains and field isolates from the state of Amazonas, Brazil, to A. annua infusions (5 g dry leaves in 1 L of boiling water) and the drug standards chloroquine, quinine and artemisinin were evaluated. The A. annua used was cultivated in three Amazon ecosystems (várzea, terra preta de índio and terra firme) and in the city of Paulínia, state of São Paulo, Brazil. Artemisinin levels in the A. annua leaves used were 0.90-1.13% (m/m). The concentration of artemisinin in the infusions was 40-46 mg/L. Field P. falciparum isolates were resistant to chloroquine and sensitive to quinine and artemisinin. The average 50% inhibition concentration values for A. annua infusions against field isolates were 0.11-0.14 μL/mL (these infusions exhibited artemisinin concentrations of 4.7-5.6 ng/mL) and were active in vitro against P. falciparum due to their artemisinin concentration. No synergistic effect was observed for artemisinin in the infusions.
Subject: Artemisia annua L.
infusion
antiplasmodial
in vitro
field isolate
Amazon Region
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Rights: aberto
Identifier DOI: 10.1590/S0074-02762012000700004
Address: http://dx.doi.org/10.1590/S0074-02762012000700004
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700004
Date Issue: 1-Nov-2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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