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Type: Artigo de periódico
Title: Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
Author: Nascimento, Elvira Maria Mendes
Gehrke, Flávia de Sousa
Maldonado, Rosa Amélia
Colombo, Silvia
Silva, Luiz Jacintho da
Schumaker, Teresinha Tizu Sato
Abstract: Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
Subject: Brazilian spotted fever
Rickettsia rickettsii
ompB gene
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Rights: aberto
Identifier DOI: 10.1590/S0074-02762005000300011
Date Issue: 1-May-2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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