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Type: Artigo de periódico
Title: Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay
Author: Machado, Eleuza Rodrigues
Ueta, Marlene Tiduko
Gonçalves-Pires, Maria do Rosário de Fátima
Oliveira, João Batista Alves de
Faccioli, Lúcia Helena
Costa-Cruz, Julia Maria
Abstract: The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was > 80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
Subject: Strongyloides venezuelensis
enzyme-linked immunosorbent assay
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Rights: aberto
Identifier DOI: 10.1590/S0074-02762003000600024
Date Issue: 1-Sep-2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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