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Type: Artigo de periódico
Title: IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in Campinas, Brazil: evidence of intercontinental distribution of strains
Author: Calusni, Ana Lucia Roscani
Roscani, Gláucia Nogueira
Villares, Maria Cecília Barisson
Soini, Hanna
Graviss, Edward A
Ramos, Marcelo de Carvalho
Abstract: Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis, the distribution and clustering of strains. IS6110 DNA fingerprinting was performed on a sample of M. tuberculosis isolates from patients with AFB smear-positive pulmonary TB, at a hospital in Brazil. The IS6110 profiles were analyzed and compared to a M. tuberculosis database of the Houston Tuberculosis Initiative, Houston, US. Seventy-six fingerprints were obtained from 98 patients. All M. tuberculosis strains had an IS6110 copy number between 5-21 allowing for differentiation of the isolates. Human immunodeficiency virus infection was confirmed in nearly half the patients of whom data was available. Fifty-eight strains had unique patterns, while 17 strains were grouped in 7 clusters (2 to 6 strains). When compared to the HTI database, 6 strains matched isolates from El Paso, Ciudad de Juarez, Houston, and New York. Recently acquired infections were documented in 19% of cases. The community transmission of infection is intense, since some clustered strains were recovered during the four-year study period. The intercontinental dissemination of M. tuberculosis strains is suspected by demonstration of identical fingerprints in a distant country.
Subject: tuberculosis
Mycobacterium tuberculosis
IS6110 restriction fragment length polymorphism
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Rights: aberto
Identifier DOI: 10.1590/S0074-02762003000500012
Date Issue: 1-Jul-2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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