Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/21597
Type: Artigo de periódico
Title: Changes in nuclear phenotypes following cold shock in Panstrongylus megistus (Burmeister)
Author: Garcia, Simone L
Mello, Maria Luiza S
Garcia, Nancy L
Rodrigues, Vera Lúcia CC
Abstract: The nuclear phenotypes of Malpighian tubule epithelial cells of 5th instar male nymphs of the blood-sucking insect Panstrongylus megistus were studied immediately after a short (1 h) cold shock at 0ºC, and 10 and 30 days later. The objective was to compare the responses to a cold shock with those known to occur after hyperthermia in order to provide insight into the cellular effect of cold in this species. Nuclei which usually exhibited a conspicuous Y chromosome chromocenter were the most frequent phenotype in control and treated specimens. Phenotypes in which the heterochromatin was unravelled, or in which there was nuclear fusion or cell death were more abundant in the shocked specimens. Most of the changes detected have also been found in heat-shocked nymphs, except for nuclear fusion which generates giant nuclei and which appeared to be less effective or necessary than that elicited after heat shock. Since other studies showed that a short cold shock does not affect the survival of more than 14% of 5th instar nymphs of P. megistus with domestic habit and can induce tolerance to a prolonged cold shock, heat shock proteins proteins are probably the best candidates for effective protection of the cells and the insects from drastic damage caused by low temperature shocks.
Subject: Panstrongylus megistus
cold shock
nuclear phenotypes
cell survival
cell death
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Rights: aberto
Identifier DOI: 10.1590/S0074-02762000000600027
Address: http://dx.doi.org/10.1590/S0074-02762000000600027
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600027
Date Issue: 1-Dec-2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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